dc.creator | Balatsos, N. A. A. | en |
dc.creator | Nilsson, P. | en |
dc.creator | Mazza, C. | en |
dc.creator | Cusack, S. | en |
dc.creator | Virtanen, A. | en |
dc.date.accessioned | 2015-11-23T10:23:31Z | |
dc.date.available | 2015-11-23T10:23:31Z | |
dc.date.issued | 2006 | |
dc.identifier | 10.1074/jbc.M508590200 | |
dc.identifier.issn | 219258 | |
dc.identifier.uri | http://hdl.handle.net/11615/26128 | |
dc.description.abstract | Poly(A)-specific ribonuclease (PARN) is a cap-interacting and poly(A)-specific 3′-exoribonuclease. Here we have investigated how the cap binding complex (CBC) affects human PARN activity. We showed that CBC, via its 80-kDa subunit (CBP80), inhibited PARN, suggesting that CBC can regulate mRNA deadenylation. The CBC-mediated inhibition of PARN was cap-independent, and in keeping with this, the CBP80 subunit alone inhibited PARN. Our data suggested a new function for CBC, identified CBC as a potential regulator of PARN, and emphasized the importance of communication between the two extreme ends of the mRNA as a key strategy to regulate mRNA degradation. Based on our data, we have proposed a model for CBC-mediated regulation of PARN, which relies on an interaction between CBP80 and PARN. Association of CBC with PARN might have importance in the regulated recruitment of PARN to the nonsense-mediated decay pathway during the pioneer round of translation. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc. | en |
dc.source | Journal of Biological Chemistry | en |
dc.source.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-33645240283&partnerID=40&md5=beacffd62f2f0b38f16b890bfbf4eab7 | |
dc.subject | Binding energy | en |
dc.subject | Biomedical engineering | en |
dc.subject | Data acquisition | en |
dc.subject | Enzyme inhibition | en |
dc.subject | Cap binding complex (CBC) | en |
dc.subject | Deadenylation | en |
dc.subject | Poly(A)-specific ribonuclease (PARN) | en |
dc.subject | RNA | en |
dc.subject | initiation factor 4E | en |
dc.subject | messenger RNA | en |
dc.subject | nuclear cap binding protein | en |
dc.subject | polyadenylate specific ribonuclease | en |
dc.subject | protein subunit | en |
dc.subject | ribonuclease | en |
dc.subject | unclassified drug | en |
dc.subject | capped RNA | en |
dc.subject | exoribonuclease | en |
dc.subject | poly(A)-specific ribonuclease | en |
dc.subject | adenylation | en |
dc.subject | article | en |
dc.subject | enzyme activity | en |
dc.subject | in vitro study | en |
dc.subject | priority journal | en |
dc.subject | protein function | en |
dc.subject | protein protein interaction | en |
dc.subject | RNA degradation | en |
dc.subject | RNA translation | en |
dc.subject | translation regulation | en |
dc.subject | drug antagonism | en |
dc.subject | human | en |
dc.subject | metabolism | en |
dc.subject | physiology | en |
dc.subject | polyadenylation | en |
dc.subject | Exoribonucleases | en |
dc.subject | Humans | en |
dc.subject | Nuclear Cap-Binding Protein Complex | en |
dc.subject | Protein Subunits | en |
dc.subject | RNA Caps | en |
dc.subject | RNA, Messenger | en |
dc.title | Inhibition of mRNA deadenylation by the nuclear cap binding complex (CBC) | en |
dc.type | journalArticle | en |