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dc.creatorApostolou, P.en
dc.creatorToloudi, M.en
dc.creatorIoannou, E.en
dc.creatorKourtidou, E.en
dc.creatorChatziioannou, M.en
dc.creatorKopic, A.en
dc.creatorKomiotis, D.en
dc.creatorKiritsis, C.en
dc.creatorManta, S.en
dc.creatorPapasotiriou, I.en
dc.date.accessioned2015-11-23T10:22:41Z
dc.date.available2015-11-23T10:22:41Z
dc.date.issued2013
dc.identifier10.3109/10799893.2013.828072
dc.identifier.issn1079-9893
dc.identifier.urihttp://hdl.handle.net/11615/25733
dc.description.abstractContext: The Notch signaling pathway is one of the most important pathways during normal development and implicated in self-renewal of adult stem cells and differentiation of progenitor cells. Abnormal expression of Notch receptors has been associated with many epithelial metaplastic and neoplastic lesions. Objective-materials and methods: In this particular study, it was determined the relative gene expression of Notch receptors after knockdown experiments in colon cancer stem cells (CSCs) and the gene expression changes in stemness transcription factors (Oct4, Sox2, Nanog), as well in dipeptidylpeptidase-4, CD44 antigen, Met proto-oncogene and in Metnase transposase. Results: In control CSCs Notch-2 had the higher expression, followed by Notch-1, Notch-3. Notch-4 demonstrated the lower gene expression among the receptors. The suppression of Notch-1 led to increased expression of Oct4 and Sox2, but in decreased gene expression of cMET, Setmar and CD44. The CD26 expression remained unchanged. The knockdown of Notch-2 led to decreased expression of all transcription factors. Notch-3 down regulation caused increased Oct4 gene expression, but decreased levels for the rest of the genes. Finally, the suppression of Notch-4 had the same effect as in receptor Notch-3. Discussion and conclusion: The above experimental data suggest the possible interaction between the four different receptors of Notch signaling pathway. The expression of CD26, cMET and N-methyltransferase Setmar was also changed. Finally, the stemness phenotype was changed in a different way each time, according to the receptor that was down regulated. All Notch receptors and particularly Notch-2 seem to play an important role in cancer stem cells.en
dc.sourceJournal of Receptors and Signal Transductionen
dc.source.uri<Go to ISI>://WOS:000328121600005
dc.subjectCD26en
dc.subjectCD44en
dc.subjectcMET proto-oncogeneen
dc.subjectcolon cancer stem cellsen
dc.subjectNotchen
dc.subjectsignaling pathwayen
dc.subjecttransposasesen
dc.subjectCOLORECTAL-CANCERen
dc.subjectSIGNALING PATHWAYen
dc.subjectGENE-EXPRESSIONen
dc.subjectTARGETING NOTCHen
dc.subjectNANOGen
dc.subjectCARCINOMAen
dc.subjectTHERAPYen
dc.subjectACTIVATIONen
dc.subjectRESISTANCEen
dc.subjectMUTATIONSen
dc.subjectBiochemistry & Molecular Biologyen
dc.subjectCell Biologyen
dc.titleStudy of the interaction among Notch pathway receptors, correlation with stemness, as well as their interaction with CD44, dipeptidyl peptidase-IV, hepatocyte growth factor receptor and the SETMAR transferase, in colon cancer stem cellsen
dc.typejournalArticleen


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