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Direct extraction and molecular characterization of enteroviruses genomes from human faecal samples
(2008)
Routine diagnosis of acute flaccid paralysis (AFP) is still based on classical virological procedures. Several enteroviruses serotypes are not easily isolated in cell cultures system used and routinely more than one passage ...
A comparative amplification of five different genomic regions on Coxsackie A and B viruses. Implications in clinical diagnostics
(2005)
Modern molecular approaches in Human Enterovirus detection rely on the designing of generic and often degenerate primers in order to amplify specific sequences within the enterovirus genome. In the present study a comparative ...
Identification of the commonest cystic fibrosis transmembrane regulator gene Delta F508 mutation: evaluation of PCR-single-strand conformational polymorphism and polyacrylamide gel electrophoresis
(2006)
In the present study we investigated whether single-strand conformational polymorphism (SSCP) and polyacrylamide gel electrophoresis (PAGE) could be used for the identification of the CFTR Delta F508 gene mutation, which ...
Interplay between protein carbonylation and nitrosylation in plants
(2013)
ROS and reactive nitrogen species (RNS) are key regulators of redox homeostasis in living organisms including plants. As control of redox homeostasis plays a central function in plant biology, redox proteomics could help ...
Molecular identification of enteroviruses responsible for an outbreak of aseptic meningitis; implications in clinical practice and epidemiology
(2004)
An outbreak of aseptic meningitis was recorded in Greece during the year 2001. Detection of the clinical strains was achieved by performing reverse transcription-polymerase chain reaction (RT-PCR) on RNA isolated from cell ...
Amplification of Echoviruses genomic regions by different RT-PCR protocols - a comparative study
(2004)
In the present report, the results of a comparative study in the detection of all Echoviruses reference strains as well as of 38 clinical isolates are presented. Using RT-PCR with already published primer pairs (UG(52)-UC53, ...
High sequence divergence in the 5 ' non-coding region of reference Coxsackie B and ECHO viral strains and clinical isolates revealed by restriction fragment length polymorphism analysis
(2001)
We report the restriction fragment length polymorphism (RFLP) patterns of a 440-bp-long 5' non-coding region (5'NCR) amplification target of all 34 reference Coxsackie B and ECHO (enteric cytopathic human orphan) enterovirus ...
Proteomics in the fruit tree science arena: New insights into fruit defense, development, and ripening
(2013)
Fruit tree crops are agricultural commodities of high economic importance, while fruits also represent one of the most vital components of the human diet. Therefore, a great effort has been made to understand the molecular ...
Heparan sulfate: biological significance, tools for biochemical analysis and structural characterization
(2011)
Heparan sulfate (HS) and heparin (HP) are functionally important glycosaminoglycans, which interact with a plethora of proteins and participate in several cellular events. They form specific proteoglycans, which are ...
Monitoring serum chondroitin sulfate levels in patients submitted to coronary artery bypass surgery
(2011)
Glycosaminoglycans (GAGs) are functionally important molecules of the arterial wall and play a crucial role in atherogenesis. Chondroitin sulfate/dermatan sulfate proteoglycans (CS/DSPGs) participate in several biological ...