Πλοήγηση ανά Θέμα "PCR"
Αποτελέσματα 1-20 από 47
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Application of random amplified polymorphic DNA (RAPD) markers to evaluate intraspecific genetic variation in red mullet (Mullus barbatus)
(1998)The random amplified polymorphic DNA (RAPD) technique was used to evaluate genetic affinities among eight red mullet (Mullus barbatus L., 1758) samples from the Mediterranean Sea. Twenty-nine random primers were used. ... -
Application of rpoB sequence similarity analysis, REP-PCR and BOX-PCR for the differentiation of species within the genus Geobacillus
(2008)Aim: To investigate the applicability of rpoB gene, which encodes the beta subunit of RNA polymerase, to be used as an alternative to 16S rRNA for sequence similarity analysis in the thermophilic genus Geobacillus. Rapid ... -
Assessment of genetic structure of Greek brown hare (Lepus europaeus) populations based on variation in random amplified polymorphic DNA (RAPD)
(2002)The RAPD method was used to assess the genetic differentiation of brown hare (Lepus europaeus) populations from Central Greece. Greek wild populations were compared with samples from Austria, Poland, Germany, France, and ... -
Attempted discrimination of Myzus persicae and Myzus nicotianae (Homoptera : Aphididae) by random amplified polymorphic DNA polymerase chain reaction technique
(1998)Random amplified polymorphic DNA (RAPD) analysis was applied to several clones of the Myzus persicae (Sulzer) group from Greece to assess genetic markers that could distinguish the tobacco feeding clones from those feeding ... -
Bacillus cereus: an important foodborne pathogen
(2010)B. cereus is a spore-forming bacterium, frequently found in the environment. Most of the strains can grow at a temperature range of 10 degrees - 42 degrees C. B. cereus grows under aerobic conditions, but anaerobic growth ... -
Comparison of Greek breeds of horses using RAPD markers
(2001)To assess genetic markers able to distinguish between five Greek breeds of horses, the random amplified polymorphic DNA (RAPD) technique was used. A total of 40 primers were initially screened in all breeds to identify ... -
Comparison of the Growth Curves of Cancer Cells and Cancer Stem Cells
(2014)A fundamental problem in cancer research is identification of the cells responsible for tumor formation. The latest field of cancer research has revealed the existence and role of cancer stem cells (CSCs). These findings ... -
Correlation between radiation-induced telomerase activity and human telomerase reverse transcriptase mRNA expression in HeLa cells
(2006)Purpose: To quantify and correlate human telomerase reverse transcriptase (hTERT) mRNA expression with telomerase activity (TA) after ionizing irradiation of HeLa cells. Materials and methods: TA and hTERT mRNA expression ... -
A cross-sectional study of Leishmania spp. infection in clinically healthy dogs with polymerase chain reaction and serology in Greece
(2002)A total of 73 clinically healthy hunting dogs, experiencing an outdoor lifestyle and originating from an area where canine leishmaniasis is endemic, were included in the study. Polymerase chain reaction (PCR) and indirect ... -
Current clinical, laboratory, and treatment outcome characteristics of visceral leishmaniasis: results from a seven-year retrospective study in Greece
(2015)Objectives: Visceral leishmaniasis (VL) is re-emerging in endemic areas. The epidemiological, clinical, laboratory, and treatment outcome characteristics in a large cohort of VL patients is described herein. Methods: The ... -
Cytological and molecular detection of Leishmania infantum in different tissues of clinically normal and sick cats
(2014)Natural infection of domestic cats by Leishmania infantum (synonym: L chagasi) has been demonstrated in several European, Latin American, and Asian countries, and the estimated prevalence of infection, based mainly on blood ... -
Detection and characterization of phytoplasmas infecting tomato plants in Greece
(2007)Over the course of the 2005 and 2006 growing seasons, surveys were carried out in tomato fields in different parts of Greece in order to determine the occurrence and distribution of phytoplasma diseases of tomato, and to ... -
Detection of Tritrichomonas foetus in cats in Greece
(2010)Intestinal infection of cats with Tritrichomonas foetus has been reported in the USA, Canada, several European countries, and Australia. However, T foetus has not been previously reported in cats in Greece. The aim of this ... -
Development of a single-tube polymerase chain reaction assay for the simultaneous detection of Haemophilus influenzae, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus spp. directly in clinical samples
(2009)This study describes the development and evaluation of a multiplex single-tube polymerase chain reaction assay for the simultaneous detection of Haemophilus influenzae, Pseudomonas aeruginosa, Staphylococcus aureus, and ... -
Efficacy of rifampicin in the treatment of experimental acute canine monocytic ehrlichiosis
(2013)To assess the efficacy of rifampicin in achieving clinical and haematological recovery and clearing infection in dogs with experimentally induced acute monocytic ehrlichiosis. Five Ehrlichia canis-infected Beagle dogs were ... -
European brown hare syndrome in wild European brown hares from Greece
(2005)From 1999 to mid-2003, 97 European brown hares (Lepus europaeus) found dead throughout Greece were examined by necropsy, histopathology, and reverse-transcription polymerase chain reaction (RT-PCR) for the presence of ... -
Evaluation of a serum-based PCR assay for the diagnosis of canine monocytic ehrlichiosis
(2009)The aim of this study was to estimate the relative diagnostic sensitivity and specificity of a polymerase chain reaction (PCR) assay in the serum of dogs with naturally occurring non-myelosuppressive canine monocytic ...