Sfoglia per Soggetto "16S rRNA"
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Apparent richness and community composition of Bacteria and Archaea in geothermal springs
(2009)The archaeal and bacterial 16S rRNA gene diversities of the Polihnitos (89 degrees C), Edipsos (81.8 degrees C), Thermopiles (38.9 degrees C), Eleftheres (41.2 degrees C) and Lagadas (35.2 degrees C) geothermal springs in ... -
Application of rpoB sequence similarity analysis, REP-PCR and BOX-PCR for the differentiation of species within the genus Geobacillus
(2008)Aim: To investigate the applicability of rpoB gene, which encodes the beta subunit of RNA polymerase, to be used as an alternative to 16S rRNA for sequence similarity analysis in the thermophilic genus Geobacillus. Rapid ... -
Changes of the bacterial assemblages throughout an urban drinking water distribution system
(2010)We analyzed the bacterial 16S rRNA gene diversity throughout the major components of the drinking water distribution system of a ca. 52,000-inhabitants city (Trikala City, Greece) in order to describe the changes of the ... -
Comparison of the Norway lobster (Nephrops norvegicus) gut bacterial communities using 16S rDNA clone libraries and pyrosequencing
(2013)By comparing 16S rDNA cloning and 454 pyrosequencing in the Nephrops norvegicus midgut, several common bacterial OTUs were detected. However, when only one method is to be selected, it needs to be considered whether the ... -
Dietary differences are reflected on the gut prokaryotic community structure of wild and commercially reared sea bream (Sparus aurata)
(2014)We compared the gut prokaryotic communities in wild, organically-, and conventionally reared sea bream (Sparus aurata) individuals. Gut microbial communities were identified using tag pyrosequencing of the 16S rRNA genes. ... -
Diversity of cyanobacterial phylotypes in a Mediterranean drinking water reservoir (Marathonas, Greece)
(2011)The structure of the cyanobacterial community in a large drinking water reservoir (Marathonas, Greece) was investigated in October 2007 and September 2008. Cyanobacteria-specific primers were used for the PCR amplification ... -
Evaluation of a serum-based PCR assay for the diagnosis of canine monocytic ehrlichiosis
(2009)The aim of this study was to estimate the relative diagnostic sensitivity and specificity of a polymerase chain reaction (PCR) assay in the serum of dogs with naturally occurring non-myelosuppressive canine monocytic ... -
Genetic variation among Mediterranean populations of Sesamia nonagrioides (Lepidoptera : Noctuidae) as revealed by RFLP mtDNA analysis
(2007)Restriction fragment length polymorphism analysis of two segments of mitochondrial DNA (COI and 16S rRNA) was used to examine genetic variation in Sesamia nonagrioides (Lefebvre) populations from the Mediterranean basin. ... -
Indigenous and spoilage microbiota of farmed sea bream stored in ice identified by phenotypic and 16S rRNA gene analysis
(2013)Investigation of the initial and spoilage microbial diversity of iced stored sea bream was carried out. Culture dependent methods were used for bacterial enumeration and phenotypic identification of bacterial isolates, ... -
Low Bacterial Diversity and High Labile Organic Matter Concentrations in the Sediments of the Medee Deep-Sea Hypersaline Anoxic Basin
(2012)Studies in the center and margin of the Medee Basin, a Mediterranean deep-sea hypersaline anoxic basin, and at a reference site during Penelope cruise (2007), revealed the existence of a 7 m-thick halocline, with high ... -
Microbiological changes, shelf life and identification of initial and spoilage microbiota of sea bream fillets stored under various conditions using 16S rRNA gene analysis
(2015)BACKGROUNDSea bream fillets are one of the most important value-added products of the seafood market. Fresh seafood spoils mainly owing to bacterial action. In this study an exploration of initial and spoilage microbiota ... -
Molecular detection of potentially toxic cyanobacteria and their associated bacteria in lake water column and sediment
(2010)We investigated the molecular diversity of cyanobacteria and bacteria during a water bloom in a lake with a long history of toxic cyanobacterial blooms (Lake Kastoria, Greece). We also tested the hypothesis whether ... -
Monitoring of spoilage and determination of microbial communities based on 16S rRNA gene sequence analysis of whole sea bream stored at various temperatures
(2016)Exploration of initial and spoilage microbiota grown on plates of whole sea bream stored aerobically at 0 (ice), 5 and 15 °C, was conducted by 16S rRNA gene sequence analysis. The course of spoilage was recorded by monitoring ... -
New findings on the true-branched monotypic genus Iphinoe (Cyanobacteria) from geographically isolated caves (Greece)
(2013)The type of branching is a morphological character of great significance; separating taxa within the true-branched cyanobacteria. In this paper, true-branched morphospecies of cyanobacteria belonging to genus Iphinoe ... -
A new set of 16S rRNA universal primers for identification of animal species
(2014)In this study, bioinformatics were used to specifically design universal primers within 16S rRNA gene according to the following criteria: the priming sites needed to be sufficiently conserved to permit a reliable amplification ... -
Raphidiopsis mediterranea Skuja represents non-heterocytous life-cycle stages of Cylindrospermopsis raciborskii (Woloszynska) Seenayya et Subba Raju in Lake Kastoria (Greece), its type locality: Evidence by morphological and phylogenetic analysis
(2009)The taxonomical relationship of Cylindrospermopsis raciborskii and Raphidiopsis mediterranea was studied by morphological and 16S rRNA gene diversity analyses of natural populations from Lake Kastoria, Greece. Samples were ... -
Selective isolation of indigenous Pseudomonas syringae strains with ice nucleation activity properties from a ski resort
(2011)Four sampling sites were selected in the area of Velouhi mountain, Greece in order to screen for Pseudomonas syringae isolates with high ice nucleation activity from a ski resort environment. Bacterial isolates (n = 147) ... -
Variability of Prokaryotic Community Structure in a Drinking Water Reservoir (Marathonas, Greece)
(2012)The structure of the Bacteria and Archaea community in a large drinking water reservoir (Marathonas, Greece; MR) was investigated in October 2007 and September 2008, using 16S rRNA gene clone libraries. The bacterial ...