Πλοήγηση ανά Θέμα "Biochemical Research Methods"
Αποτελέσματα 21-40 από 53
-
High sequence divergence in the 5 ' non-coding region of reference Coxsackie B and ECHO viral strains and clinical isolates revealed by restriction fragment length polymorphism analysis
(2001)We report the restriction fragment length polymorphism (RFLP) patterns of a 440-bp-long 5' non-coding region (5'NCR) amplification target of all 34 reference Coxsackie B and ECHO (enteric cytopathic human orphan) enterovirus ... -
High-performance liquid chromatographic determination of benzoylurea insecticides residues in grapes and wine using liquid and solid-phase extraction
(1999)A method for the determination of the benzoylurea insecticides diflubenzuron, triflumuron, teflubenzuron, lufenuron and flufenoxuron in grapes and wine by HPLC has been developed and validated. Grape samples (50 g) were ... -
Identification of red natural dyes in post-byzantine icons by HPLC
(2005)A high performance liquid chromatography (HPLC) methodology, combined with UV-Vis Diode Array Detection, is developed for the separation and identification of five reddish natural dyestuffs: cochineal, madder, lac dye, ... -
Identification of the commonest cystic fibrosis transmembrane regulator gene Delta F508 mutation: evaluation of PCR-single-strand conformational polymorphism and polyacrylamide gel electrophoresis
(2006)In the present study we investigated whether single-strand conformational polymorphism (SSCP) and polyacrylamide gel electrophoresis (PAGE) could be used for the identification of the CFTR Delta F508 gene mutation, which ... -
Importance of high-performance liquid chromatographic analysis of serum N-acylneuraminic acids in evaluating surgical treatment in patients with early endometrial cancer
(1999)The objectives of this study were the quantification of the two major sialic acid (Sia) forms - N-acetylneuraminic (Neu5Ac) and N-glycolylneuraminic acids (Neu5Gc) - in serum before and after surgical treatment of early ... -
In vivo evaluation of CYP1A2 CYP2A6, NAT-2 and xanthine oxidase activities in a Greek population sample by the RP-HPLC monitoring of caffeine metabolic ratios
(2007)A RP-HPLC method was developed for the assessment of caffeine and its metabolites in urine and was used for the evaluation of the CYP1A2, CYP2A6, xanthine oxidase (XO) and N-acetyl-transferase-2 (NAT-2) in vivo activities ... -
Inhibitor design for ribonuclease A: the binding of two 5 '-phosphate uridine analogues
(2009)In the quest for the rational design of selective and potent inhibitors for members of the pancreatic ribonuclease A (RNase A) family of biomedical interest, the binding of uridine 5'-phosphate (U5P) and uridine 5'-diphosphate ... -
Interplay between protein carbonylation and nitrosylation in plants
(2013)ROS and reactive nitrogen species (RNS) are key regulators of redox homeostasis in living organisms including plants. As control of redox homeostasis plays a central function in plant biology, redox proteomics could help ... -
KEGGconverter: a tool for the in-silico modelling of metabolic networks of the KEGG Pathways database
(2009)Background: The KEGG Pathway database is a valuable collection of metabolic pathway maps. Nevertheless, the production of simulation capable metabolic networks from KEGG Pathway data is a challenging complicated work, ... -
Laboratory diagnosis of contagious ecthyma: Comparison of different PCR protocols with virus isolation in cell culture
(2006)A new polymerase chain reaction (PCR) assay for rapid diagnosis of contagious ecthyma was designed and applied to 21 clinical samples from Greece. This assay, which detects a highly conserved gene from the parapox genome, ... -
Microwave-assisted Extraction of Glucosinolates from Eruca sativa Seeds and Soil: Comparison with Existing Methods
(2009)Introduction - Glucosinolates (GSLs) are secondary plant metabolites that are abundant in brassicas and their hydrolysis products, isothiocyanates, are toxic to soil pathogens. Efficiency and extraction time are critical ... -
mirPub: a database for searching microRNA publications
(2015)A Summary: Identifying, amongst millions of publications available in MEDLINE, those that are relevant to specific microRNAs (miRNAs) of interest based on keyword search faces major obstacles. References to miRNA names in ... -
Molecular diagnostic tools in mycobacteriology
(2008)Although the diagnosis of mycobacteriosis and susceptibility testing are still primarily based on conventional methods (staining, culture, biochemical analysis, proportional method), a series of molecular assays are ... -
Molecular identification of enteroviruses responsible for an outbreak of aseptic meningitis; implications in clinical practice and epidemiology
(2004)An outbreak of aseptic meningitis was recorded in Greece during the year 2001. Detection of the clinical strains was achieved by performing reverse transcription-polymerase chain reaction (RT-PCR) on RNA isolated from cell ... -
Monitoring serum chondroitin sulfate levels in patients submitted to coronary artery bypass surgery
(2011)Glycosaminoglycans (GAGs) are functionally important molecules of the arterial wall and play a crucial role in atherogenesis. Chondroitin sulfate/dermatan sulfate proteoglycans (CS/DSPGs) participate in several biological ... -
A multi-residue method for pesticide residue analysis in rice grains using matrix solid-phase dispersion extraction and high-performance liquid chromatography-diode array detection
(2010)Pesticides are widely used in rice cultivation, often resulting in detection of their residues in rice grains. So far, no analytical method has been available for the simultaneous determination of most rice pesticides in ... -
A new RT-PCR assay for the identification of the predominant recombination types in 2C and 3D genomic regions of vaccine-derived poliovirus strains
(2010)In the post-eradication era of wild polioviruses, the only remaining sources of poliovirus infection worldwide would be the vaccine-derived polioviruses (VDPVs). As the preponderance of countries certified to be polio-free ... -
Optimization of TNF-alpha, overexpression in Escherichia coli using response surface methodology: Purification of the protein and oligomerization studies
(2012)Tumor necrosis factor-alpha (TNF-alpha) is responsible for many autoimmune disorders including rheumatoid arthritis, psoriasis, Chron's disease, stroke, and atherosclerosis. Thus, inhibition of TNF-alpha is a major challenge ... -
PCR assays for the identification of rare recombination types from VP1 to 3D genomic region of vaccine derived poliovirus strains
(2014)Poliomyelitis has been effectively controlled by the use of inactivated poliovirus vaccine (IPV) or trivalent live attenuated oral poliovirus vaccine (OPV). Since 1964, the use of OPV in mass vaccinations has resulted in ... -
Plant GSTome: structure and functional role in xenome network and plant stress response
(2015)Glutathione transferases (GSTs) represent a major group of detoxification enzymes. All plants possess multiple cytosolic GSTs, each of which displays distinct catalytic as well as non-catalytic binding properties. The ...