Browsing by Subject "Biochemical Research Methods"
Now showing items 1-20 of 53
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Amplification of Echoviruses genomic regions by different RT-PCR protocols - a comparative study
(2004)In the present report, the results of a comparative study in the detection of all Echoviruses reference strains as well as of 38 clinical isolates are presented. Using RT-PCR with already published primer pairs (UG(52)-UC53, ... -
Application of single-drop microextraction coupled with gas chromatography for the determination of multiclass pesticides in vegetables with nitrogen phosphorus and electron capture detection
(2009)In the present work the single-drop microextraction (SDME) technique coupled with GC-NPD and GC-ECD was evaluated for the determination of multi-class pesticides in vegetables. The donor sample solution preparation was ... -
Characterization of novel intergenogroup and intergenotype recombinant noroviruses from central Greece
(2014)Noroviruses (NoVs) are a major causative agent of acute gastroenteritis in humans. They are members of the Caliciviridae family and based on the genetic analysis of the RdRp and capsid regions, human NoVs are divided into ... -
Chemometric study and optimization of extraction parameters in single-drop microextraction for the determination of multiclass pesticide residues in grapes and apples by gas chromatography mass spectrometry
(2009)A simple and rapid single-drop microextraction method coupled with gas chromatography and mass spectrometry (SDME-GC/MS) for the determination of 20 pesticides with different physicochemical properties in grapes and apples ... -
Combined prediction of Tat and Sec signal peptides with hidden Markov models
(2010)Motivation: Computational prediction of signal peptides is of great importance in computational biology. In addition to the general secretory pathway ( Sec), Bacteria, Archaea and chloroplasts possess another major pathway ... -
A comparative amplification of five different genomic regions on Coxsackie A and B viruses. Implications in clinical diagnostics
(2005)Modern molecular approaches in Human Enterovirus detection rely on the designing of generic and often degenerate primers in order to amplify specific sequences within the enterovirus genome. In the present study a comparative ... -
Development and application of GC-MS method for monitoring of long-term exposure to the pesticide cypermethrin
(2014)Cypermethrin (CPMN) is a synthetic pyrethroid used as an insecticide in large-scale commercial agricultural applications as well as for domestic purposes. In the present study a gas chromatography-mass spectrometry (GC-MS) ... -
Diagnostic performance of a rapid in-clinic test for the detection of Canine Parvovirus under different storage conditions and vaccination status
(2015)Canine parvovirus (CPV) is one of the most common causes of acute haemorrhagic enteritis in young dogs, while clinical diagnosis is often indecisive. The aim of our study was to evaluate the diagnostic accuracy of an ... -
Direct extraction and molecular characterization of enteroviruses genomes from human faecal samples
(2008)Routine diagnosis of acute flaccid paralysis (AFP) is still based on classical virological procedures. Several enteroviruses serotypes are not easily isolated in cell cultures system used and routinely more than one passage ... -
Duplex Real-time PCR assay and SYBR green I melting curve analysis for molecular identification of HPV genotypes 16, 18, 31, 35, 51 and 66
(2015)Long-term infection with high-risk HPV genotypes is the leading cause of cervical cancer. In the present study a Duplex Real-time PCR assay was developed in order to identify HPV types 16, 18, 31, 35, 51 and 66 in three ... -
Efficient soluble expression of active recombinant human cyclin A2 mediated by E. coli molecular chaperones
(2015)Bacterial expression of human proteins continues to present a critical challenge in protein crystallography and drug design. While human cyclin A constructs have been extensively characterized in complex with cyclin dependent ... -
Evaluation and Properties of the Budding Yeast Phosphoproteome
(2012)We have assembled a reliable phosphoproteomic data set for budding yeast Saccharomyces cerevisiae and have investigated its properties. Twelve publicly available phosphoproteome data sets were triaged to obtain a subset ... -
Evaluation of a microsphere-based flow cytometric assay for diagnosis of Celiac disease
(2004)The multiplexed particle-based flow cytometric technology proposes a new approach for the diagnosis of autoimmune diseases combining the advantages of conventional methods with the ability to quantitatively determine ... -
Evaluation of a novel method based on PCR Restriction Fragment Length Polymorphism Analysis of the tuf gene for the identification of Staphylococcus species
(2003)A novel method, based on PCR Restriction Fragment Length Polymorphism Analysis (PRA) of a part of the tuf gene (370 bp), was designed for the identification of 11 staphylococcal species, including the most common staphylococcal ... -
ExTopoDB: a database of experimentally derived topological models of transmembrane proteins
(2010)ExTopoDB is a publicly accessible database of experimentally derived topological models of transmembrane proteins. It contains information collected from studies in the literature that report the use of biochemical methods ... -
Gas chromatographic - Mass spectrometric cardiotonic glycosides detection in equine urine doping analysis
(2004)A screening method for the detection of cardiotonic glycosides in horse's urine is presented in this paper. This method is based on the detection by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-high ... -
GC-MS analysis of blood for the metabonomic investigation of the effects of physical exercise and allopurinol administration on rats
(2014)Exhaustive exercise is a generator of free radicals and reactive species in mammals. Allopurinol is a known inhibitor of xanthine oxidase, a source of free radicals during exercise. In this study, the influence of allopurinol ... -
gpDB: a database of GPCRs, G-proteins, effectors and their interactions
(2008)gpDB is a publicly accessible, relational database, containing information about G-proteins, G-protein coupled receptors (GPCRs) and effectors, as well as information concerning known interactions between these molecules. ... -
HEGESMA: genome search meta-analysis and heterogeneity testing
(2005)Heterogeneity and genome search meta-analysis (HEGESMA) is a comprehensive software for performing genome scan meta-analysis, a quantitative method to identify genetic regions (bins) with consistently increased linkage ... -
Heparan sulfate: biological significance, tools for biochemical analysis and structural characterization
(2011)Heparan sulfate (HS) and heparin (HP) are functionally important glycosaminoglycans, which interact with a plethora of proteins and participate in several cellular events. They form specific proteoglycans, which are ...