dc.creator | Stefan A., Van Cauwenberghe J., Rosu C.M., Stedel C., Labrou N.E., Flemetakis E., Efrose R.C. | en |
dc.date.accessioned | 2023-01-31T10:03:17Z | |
dc.date.available | 2023-01-31T10:03:17Z | |
dc.date.issued | 2018 | |
dc.identifier | 10.1016/j.syapm.2018.01.007 | |
dc.identifier.issn | 07232020 | |
dc.identifier.uri | http://hdl.handle.net/11615/79444 | |
dc.description.abstract | The identification and conservation of indigenous rhizobia associated with legume plants and their application as biofertilizers is becoming an agricultural worldwide priority. However, little is known about the genetic diversity and phylogeny of rhizobia in Romania. In the present study, the genetic diversity and population composition of Rhizobium leguminosarum symbiovar trifolii isolates from 12 clover plants populations located across two regions in Romania were analyzed. Red clover isolates were phenotypically evaluated and genotyped by sequencing 16S rRNA gene, 16S-23S intergenic spacer, three chromosomal genes (atpD, glnII and recA) and two plasmid genes (nifH and nodA). Multilocus sequence typing (MLST) analysis revealed that red clover plants are nodulated by a wide genetic diversity of R. leguminosarum symbiovar trifolii sequence types (STs), highly similar to the ones previously found in white clover. Rhizobial genetic variation was found mainly within the two clover populations for both chromosomal and plasmid types. Many STs appear to be unique for this region and the genetic composition of rhizobia differs significantly among the clover populations. Furthermore, our results showed that both soil pH and altitude contributed to plasmid sequence type composition while differences in chromosomal composition were affected by the altitude and were strongly correlated with distance. © 2018 Elsevier GmbH | en |
dc.language.iso | en | en |
dc.source | Systematic and Applied Microbiology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041955069&doi=10.1016%2fj.syapm.2018.01.007&partnerID=40&md5=7fc24c170106b8fee5c1fab18e286f03 | |
dc.subject | RNA 16S | en |
dc.subject | spacer DNA | en |
dc.subject | bacterial DNA | en |
dc.subject | Article | en |
dc.subject | bacterial genetics | en |
dc.subject | DNA extraction | en |
dc.subject | gene amplification | en |
dc.subject | genetic isolation by distance | en |
dc.subject | genetic variability | en |
dc.subject | genotyping technique | en |
dc.subject | multilocus sequence typing | en |
dc.subject | nonhuman | en |
dc.subject | phenotype | en |
dc.subject | phylogeny | en |
dc.subject | plasmid | en |
dc.subject | priority journal | en |
dc.subject | red clover | en |
dc.subject | Rhizobium leguminosarum | en |
dc.subject | Romania | en |
dc.subject | white clover | en |
dc.subject | altitude | en |
dc.subject | bacterial gene | en |
dc.subject | chemistry | en |
dc.subject | clover | en |
dc.subject | DNA sequence | en |
dc.subject | genetic variation | en |
dc.subject | genetics | en |
dc.subject | Medicago | en |
dc.subject | microbiology | en |
dc.subject | nodulation | en |
dc.subject | phylogeny | en |
dc.subject | population genetics | en |
dc.subject | Rhizobium leguminosarum | en |
dc.subject | soil | en |
dc.subject | symbiosis | en |
dc.subject | Altitude | en |
dc.subject | DNA, Bacterial | en |
dc.subject | Genes, Bacterial | en |
dc.subject | Genetic Variation | en |
dc.subject | Genetics, Population | en |
dc.subject | Medicago | en |
dc.subject | Multilocus Sequence Typing | en |
dc.subject | Phylogeny | en |
dc.subject | Rhizobium leguminosarum | en |
dc.subject | RNA, Ribosomal, 16S | en |
dc.subject | Romania | en |
dc.subject | Root Nodules, Plant | en |
dc.subject | Sequence Analysis, DNA | en |
dc.subject | Soil | en |
dc.subject | Symbiosis | en |
dc.subject | Trifolium | en |
dc.subject | Elsevier GmbH | en |
dc.title | Genetic diversity and structure of Rhizobium leguminosarum populations associated with clover plants are influenced by local environmental variables | en |
dc.type | journalArticle | en |