Evidence of blood and muscle redox status imbalance in experimentally induced renal insufficiency in a rabbit model
Ημερομηνία
2019Γλώσσα
en
Λέξη-κλειδί
Επιτομή
Chronic kidney disease (CKD) is accompanied by a disturbed redox homeostasis, especially in end-stage patients, which is associated with pathological complications such as anemia, atherosclerosis, and muscle atrophy. However, limited evidence exists about redox disturbances before the end stage of CKD. Moreover, the available redox literature has not yet provided clear associations between circulating and tissue-specific (muscle) oxidative stress levels. The aim of the study was to evaluate commonly used redox status indices in the blood and in two different types of skeletal muscle (psoas, soleus) in the predialysis stages of CKD, using an animal model of renal insufficiency, and to investigate whether blood redox status indices could be reflecting the skeletal muscle redox status. Indices evaluated included reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione reductase (GR), catalase (CAT), total antioxidant capacity (TAC), protein carbonyls (PC), and thiobarbituric acid reactive substances (TBARS). Results showed that blood GSH was higher in the uremic group compared to the control (17 50 ± 1 73 vs. 12 43 ± 1 01, p = 0 033). In both muscle types, PC levels were higher in the uremic group compared to the control (psoas: 1 086 ± 0 294 vs. 0 596 ± 0 372, soleus: 2 52 ± 0 29 vs. 0 929 ± 0 41, p < 0 05). The soleus had higher levels of TBARS, PC, GSH, CAT, and GR and lower TAC compared to the psoas in both groups. No significant correlations in redox status indices between the blood and skeletal muscles were found. However, in the uremic group, significant correlations between the psoas and soleus muscles in PC, GSSG, and CAT levels emerged, not present in the control. Even in the early stages of CKD, a disturbance in redox homeostasis was observed, which seemed to be muscle type-specific, while blood levels of redox indices did not seem to reflect the intramuscular condition. The above results highlight the need for further research in order to identify the key mechanisms driving the onset and progression of oxidative stress and its detrimental effects on CKD patients. © 2019 Konstantina P. Poulianiti et al.
Collections
Related items
Showing items related by title, author, creator and subject.
-
Evidence of a Redox-Dependent Regulation of Immune Responses to Exercise-Induced Inflammation
Sakelliou A., Fatouros I.G., Athanailidis I., Tsoukas D., Chatzinikolaou A., Draganidis D., Jamurtas A.Z., Liacos C., Papassotiriou I., Mandalidis D., Stamatelopoulos K., Dimopoulos M.A., Mitrakou A. (2016)We used thiol-based antioxidant supplementation (n-acetylcysteine, NAC) to determine whether immune mobilisation following skeletal muscle microtrauma induced by exercise is redox-sensitive in healthy humans. According to ... -
Iron Supplementation Effects on Redox Status following Aseptic Skeletal Muscle Trauma in Adults and Children
Deli C.K., Fatouros I.G., Paschalis V., Tsiokanos A., Georgakouli K., Zalavras A., Avloniti A., Koutedakis Y., Jamurtas A.Z. (2017)Exercise-induced skeletal muscle microtrauma is characterized by loss of muscle cell integrity, marked aseptic inflammatory response, and oxidative stress. We examined if iron supplementation would alter redox status after ... -
A lightly roasted coffee extract improves blood and tissue redox status in rats through enhancement of GSH biosynthesis
Priftis A., Soursou V., Makiou A.-S., Tekos F., Veskoukis A.S., Tsantarliotou M.P., Taitzoglou I.A., Kouretas D. (2019)Coffee is a highly consumed beverage with many putative beneficial health effects, however these often come from observational studies. In the current work, a lightly roasted coffee extract that has previously been reported ...