dc.creator | Papatzimos G., Kotzamanidis C., Kyritsi M., Malissiova E., Economou V., Giantzi V., Zdragas A., Hadjichristodoulou C., Sergelidis D. | en |
dc.date.accessioned | 2023-01-31T09:45:12Z | |
dc.date.available | 2023-01-31T09:45:12Z | |
dc.date.issued | 2022 | |
dc.identifier | 10.1111/lam.13620 | |
dc.identifier.issn | 02668254 | |
dc.identifier.uri | http://hdl.handle.net/11615/77886 | |
dc.description.abstract | In this study, we investigated the incidence of Listeria monocytogenes in the receiving meat, the meat products, the personnel and the environment of a vertically integrated company in Northern Greece owing a processing plant and three trading facilities. A total of 303 samples were examined from the receiving raw meat, raw meat preparations, ready-to-eat meat products, processing surfaces and the environment of these facilities as well as the food handlers’ hands and nasal cavities. MALDI-TOF MS was used for Listeria identification; from the 22 (7·26%) positive to Listeria spp. isolates, 12 (3·96%) identified as L. monocytogenes, eight (2·64%) as Listeria innocua and two (0·66%) as Listeria welshimeri. Molecular serotyping of L. monocytogenes isolates by multiplex PCR revealed 11 strains belonging to serogroup IIa (1/2a and 3a) and one to IIc (1/2c and 3c). The assay for the detection of the virulence-associated genes revealed eight isolates carrying all the examined genes (inlA, inlB, inlC, plcA, prfA, actA, hlyA and iap) and four carrying all except the actA gene. Eleven (91·7%) of the isolates showed a strong ability to form biofilm. All isolates were multidrug resistant. The MALDI-TOF Main Spectrum Profile (MSPs), revealed three clusters: one with five isolates (four from environmental samples and one from a food handler), one with five isolates (all from environmental samples) and one with two isolates (both from raw meat products). MALDI-TOF MS seems to be a reliable tool for the identification of niches and contamination routes in processing plants, contributing also to the evaluation and improvement of the applied preventive measures to control L. monocytogenes. © 2021 The Society for Applied Microbiology | en |
dc.language.iso | en | en |
dc.source | Letters in Applied Microbiology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120815321&doi=10.1111%2flam.13620&partnerID=40&md5=64e9b77b84b385fea6388dbf9102a319 | |
dc.subject | bushmeat | en |
dc.subject | disease prevalence | en |
dc.subject | gene expression | en |
dc.subject | meat | en |
dc.subject | sampling | en |
dc.subject | antibiotic resistance | en |
dc.subject | article | en |
dc.subject | bacterial strain | en |
dc.subject | bacterial virulence | en |
dc.subject | bacterium isolate | en |
dc.subject | biofilm | en |
dc.subject | contamination | en |
dc.subject | convenience food | en |
dc.subject | Greece | en |
dc.subject | human | en |
dc.subject | incidence | en |
dc.subject | Listeria innocua | en |
dc.subject | Listeria monocytogenes | en |
dc.subject | Listeria welshimeri | en |
dc.subject | major clinical study | en |
dc.subject | matrix assisted laser desorption ionization time of flight mass spectrometry | en |
dc.subject | meat industry | en |
dc.subject | multiplex polymerase chain reaction | en |
dc.subject | nonhuman | en |
dc.subject | nose cavity | en |
dc.subject | prevalence | en |
dc.subject | raw meat | en |
dc.subject | retail outlet | en |
dc.subject | serotype | en |
dc.subject | serotyping | en |
dc.subject | food contamination | en |
dc.subject | food control | en |
dc.subject | genetics | en |
dc.subject | Greece | en |
dc.subject | meat | en |
dc.subject | prevalence | en |
dc.subject | Greece | en |
dc.subject | Food Contamination | en |
dc.subject | Food Microbiology | en |
dc.subject | Greece | en |
dc.subject | Listeria monocytogenes | en |
dc.subject | Meat | en |
dc.subject | Meat Products | en |
dc.subject | Prevalence | en |
dc.subject | John Wiley and Sons Inc | en |
dc.title | Prevalence and characteristics of Listeria monocytogenes in meat, meat products, food handlers and the environment of the meat processing and the retail facilities of a company in Northern Greece | en |
dc.type | journalArticle | en |