dc.creator | Jagirdar R.M., Apostolidou E., Molyvdas P.A., Gourgoulianis K.I., Hatzoglou C., Zarogiannis S.G. | en |
dc.date.accessioned | 2023-01-31T08:28:44Z | |
dc.date.available | 2023-01-31T08:28:44Z | |
dc.date.issued | 2016 | |
dc.identifier | 10.1152/ajplung.00410.2015 | |
dc.identifier.issn | 10400605 | |
dc.identifier.uri | http://hdl.handle.net/11615/74072 | |
dc.description.abstract | Malignant pleural mesothelioma (MPM) is an aggressive cancer. MPM cells express aquaporin-1 (AQP1) that in other cancers has been shown to participate in the tumor metastasis processes. However, in MPM patients AQP1 overexpression is an independent prognostic factor favoring survival. In this study we aimed at evaluating the role of AQP1 in cell adhesion, migration, and tumor sphere formation in nonmalignant mesothelial cells (MeT-5A) and in epithelioid (M14K) and sarcomatoid (ZL34) MPM cell lines. We used fibronectin (FN) or homologous cell-derived extracellular martrix (ECM) substratum to investigate the role of AQP1 in these experimental phenotypes, inhibiting AQP1 by 10_5 M mercury chloride (MC). Deposited ECM during cell culture exhibited significant concentration differences among cell types. ZL34 cell adhesion was significantly higher than MeT-5A or M14K cells on FN and ECM. MeT-5A and M14K cell adhesion on FN was sensitive to AQP1 inhibition, whereas AQP1 inhibition on ECM was limited to M14K cells. Wound healing in ZL34 cells was significantly higher than MeT-5A and M14K cells on FN and ECM. AQP1 inhibition significantly lowered cell migration in ZL34 cells on FN and ECM. Sphere formation was not dependent on FN or ECM in the media. AQP1 inhibition in FN media reduced sphere formation in M14K cells, whereas, in ECM, all three cell types were sensitive to AQP1 inhibition. © 2016 the American Physiological Society. | en |
dc.language.iso | en | en |
dc.source | American Journal of Physiology - Lung Cellular and Molecular Physiology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84976619972&doi=10.1152%2fajplung.00410.2015&partnerID=40&md5=df4653606bc2d567f05b39c262ae6cf3 | |
dc.subject | aquaporin 1 | en |
dc.subject | fibronectin | en |
dc.subject | mercury chloride | en |
dc.subject | AQP1 protein, human | en |
dc.subject | aquaporin 1 | en |
dc.subject | fibronectin | en |
dc.subject | mercuric chloride | en |
dc.subject | Article | en |
dc.subject | cell adhesion | en |
dc.subject | cell migration | en |
dc.subject | cell structure | en |
dc.subject | controlled study | en |
dc.subject | epithelioid cell | en |
dc.subject | extracellular matrix | en |
dc.subject | human | en |
dc.subject | human cell | en |
dc.subject | mesothelium cell | en |
dc.subject | pleura mesothelioma | en |
dc.subject | priority journal | en |
dc.subject | protein expression | en |
dc.subject | sarcomatoid carcinoma | en |
dc.subject | tumor sphere formation | en |
dc.subject | wound closure | en |
dc.subject | wound healing | en |
dc.subject | antagonists and inhibitors | en |
dc.subject | cell adhesion | en |
dc.subject | cell culture technique | en |
dc.subject | cell motion | en |
dc.subject | cell shape | en |
dc.subject | extracellular matrix | en |
dc.subject | lung tumor | en |
dc.subject | mesothelioma | en |
dc.subject | metabolism | en |
dc.subject | multicellular spheroid | en |
dc.subject | pathology | en |
dc.subject | physiology | en |
dc.subject | pleura tumor | en |
dc.subject | tumor cell line | en |
dc.subject | Aquaporin 1 | en |
dc.subject | Cell Adhesion | en |
dc.subject | Cell Culture Techniques | en |
dc.subject | Cell Line, Tumor | en |
dc.subject | Cell Movement | en |
dc.subject | Cell Shape | en |
dc.subject | Extracellular Matrix | en |
dc.subject | Fibronectins | en |
dc.subject | Humans | en |
dc.subject | Lung Neoplasms | en |
dc.subject | Mercuric Chloride | en |
dc.subject | Mesothelioma | en |
dc.subject | Pleural Neoplasms | en |
dc.subject | Spheroids, Cellular | en |
dc.subject | American Physiological Society | en |
dc.title | Influence of AQP1 on cell adhesion, migration, and tumor sphere formation in malignant pleural mesothelioma is substratum-and histological-type dependent | en |
dc.type | journalArticle | en |