Clinical, ultrasonographic, bacteriological, cytological and histopathological findings of uterine involution in ewes with uterine infection
Datum
2020Language
en
Schlagwort
Zusammenfassung
The objectives of the study were (a) to study the characteristics of uterine involution in ewes that had developed subclinical uterine infection in the immediately post-partum period and (b) to evaluate effects of the infection in the subsequent reproductive performance of ewes. Uterine infection was induced in ewes (I, n = 10) by intrauterine inoculation of Escherichia coli; uninoculated controls were included (C, n = 12). Animals were examined at regular intervals before and post-inoculation. Clinical and ultrasonographic examinations were performed. Vaginal swab samples and biopsy uterine tissue samples were collected for bacteriological, cytological and histological examination. Finally, ewes were put to rams and reproductive performance was monitored. After challenge, it was ultrasonographically found that caruncular dimensions, myometrial thickness and diameter of uterine lumen were greater in I ewes. In these ewes, particular reduction of dimensions occurred during the second week post-partum, whilst in C ewes during the first week. The uterine artery diameter and the blood flow into the uterus were also greater in I than in C ewes. E. coli infection was more frequent and of longer duration in I than in C ewes: in 68.1% and 50.0% of ewes and 19.5 and 14 days, respectively. There was lower proportion of neutrophils and higher of lymphocytes in group I than in C. In inoculated ewes, there was histological evidence of uterine epithelial destruction, increased cellular infiltration, hyperaemia and extracasation, which persisted up to 42 days post-partum. During the subsequent reproductive season, all ewes in group I lambed normally and produced healthy and viable lambs. No significant difference in reproductive performance parameters were seen in I comparison to C ewes. It is concluded that the innate immunity of the uterus sufficed to counteract the bacterial infection, although the process of involution took longer than in healthy animals; moreover, the ultrasonographic examination is a useful means for assessment of the genital tract of ewes post-partum; finally, no adverse effects were noted in the subsequent reproductive performance of ewes. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Collections
Verwandte Dokumente
Anzeige der Dokumente mit ähnlichem Titel, Autor, Urheber und Thema.
-
Quantitative and Morphological Blood Cell Findings Associated with the Presence of Antigen and/or Antibodies against Anaplasma phagocytophilum in Sheep
Katsogiannou E.G., Katsoulos P.D., Christodoulopoulos G., Polizopoulou Z.S., Papadakis S.M., Kokkinaki K.C., Billinis C., Boutsini S., Stougiou D., Athanasiou L.V. (2021)Anaplasma phagocytophilum is the causative agent of a disease known as tick borne fever in sheep, although fever is not always present. Due to inconclusive clinical signs, diagnosis is based on the cytological or molecular ... -
Blood cell count and morphology, and vitamin B12 concentration in pre- and post-weaned calves
Katsogiannou E.G., Katsoulos P.D., Ziogas C., Naskou M.C., Christodoulopoulos G., Polizopoulou Z.S., Tzivara A., Athanasiou L.V. (2021)Haematological indicators may resent physiological variation by age. Vitamin B12 promotes haematopoiesis. The aims of this study were: 1) to compare the values of the haematological variables and the concentration of vitamin ... -
Estimation of white blood cell and platelet counts in ovine blood smears, and a comparison with the ADVIA 120 hematology analyzer
Katsogiannou E.G., Athanasiou L.V., Katsoulos P.D., Polizopoulou Z.S., Tzivara A., Christodoulopoulos G. (2020)Background: Manual evaluation of blood cell counts on stained blood films is a common procedure in resource-limited laboratories of farm animal clinics. Moreover, settings for sheep blood cell counts are not provided on ...