dc.creator | Dovolou E., Samartzi F., Perreau C., Krania F., Cordova A., Vainas E., Amiridis G.S., Mermillod P., Tsiligianni T. | en |
dc.date.accessioned | 2023-01-31T07:59:00Z | |
dc.date.available | 2023-01-31T07:59:00Z | |
dc.date.issued | 2016 | |
dc.identifier | 10.1016/j.theriogenology.2016.01.003 | |
dc.identifier.issn | 0093691X | |
dc.identifier.uri | http://hdl.handle.net/11615/73455 | |
dc.description.abstract | We studied the role of follicular fluid's (FF) glycosidase (α-mannosidase [α-ΜΑΝ], β-Ν-acetyloglucosaminidase [NAGASE], β-galactosidase [β-GAL]) activity during IVM of bovine oocytes. Oocytes were allocated into two groups according to the follicular size (small follicle [SF]: 2–5 mm, large follicle [LF]: >5–8 mm). In experiment 1, cumulus–oocyte complexes (COCs) quality was evaluated according to morphologic criteria (grades A, B–C, D); oocyte (n = 801) nuclear maturation was assessed after 24 hours of incubation. Bovine embryos were produced in vitro in groups (experiment 2, n = 1503 oocytes) or individually (experiment 3, n = 50 oocytes). More grade-A and -BC COCs were collected from SF and LF groups, respectively (P < 0.05). Maturation rate (experiment 1) and cleavage rate (experiments 2 and 3) were similar in SF and LF groups. Activity of all glycosidases in FF was higher (P < 0.05) in SF group than in LF group, whereas in maturation medium of SF group it was, overall, significantly lower than in that of LF (experiments 2 and 3). In FF of SF group, NAGASE positively associated with grade-A oocytes and negatively with BC oocytes; increased β-GAL was associated with degenerated oocytes. Cleavage rate in LF group, related negatively to NAGASE and positively to α-MAN in maturation medium. These results indicate that during maturation, COCs release NAGASE and consume β-GAL, but differences probably exist between individual and group maturation. © 2016 Elsevier Inc. | en |
dc.language.iso | en | en |
dc.source | Theriogenology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84956653830&doi=10.1016%2fj.theriogenology.2016.01.003&partnerID=40&md5=ad9e44cbc528d21fe9f71cb99fddf7a7 | |
dc.subject | acetylglucosaminidase | en |
dc.subject | alpha mannosidase | en |
dc.subject | beta galactosidase | en |
dc.subject | culture medium | en |
dc.subject | animal | en |
dc.subject | bovine | en |
dc.subject | cell culture technique | en |
dc.subject | culture medium | en |
dc.subject | female | en |
dc.subject | growth, development and aging | en |
dc.subject | in vitro oocyte maturation | en |
dc.subject | metabolism | en |
dc.subject | oocyte | en |
dc.subject | ovary follicle fluid | en |
dc.subject | physiology | en |
dc.subject | procedures | en |
dc.subject | veterinary | en |
dc.subject | Acetylglucosaminidase | en |
dc.subject | alpha-Mannosidase | en |
dc.subject | Animals | en |
dc.subject | beta-Galactosidase | en |
dc.subject | Cattle | en |
dc.subject | Cell Culture Techniques | en |
dc.subject | Culture Media | en |
dc.subject | Female | en |
dc.subject | Follicular Fluid | en |
dc.subject | In Vitro Oocyte Maturation Techniques | en |
dc.subject | Oocytes | en |
dc.subject | Elsevier Inc. | en |
dc.title | The activity of three glycosidases (β-Ν-acetyloglucosaminidase, α-mannosidase, and β-galactosidase) in the follicular fluid and in the maturation medium affects bovine oocyte maturation | en |
dc.type | journalArticle | en |