Comparative Evaluation of Tigecycline Susceptibility Testing Methods for Expanded-Spectrum Cephalosporin- and Carbapenem-Resistant Gram-Negative Pathogens
Συγγραφέας
Zarkotou, O.; Pournaras, S.; Altouvas, G.; Pitiriga, V.; Tziraki, M.; Mamali, V.; Themeli-Digalaki, K.; Tsakris, A.Ημερομηνία
2012Επιτομή
We evaluated the Vitek2, Etest, and MIC Test Strip (MTS) methods of tigecycline susceptibility testing with 241 expanded-spectrum cephalosporin-resistant and/or carbapenem-resistant Enterobacteriaceae and Acinetobacter baumannii clinical isolates by using dry-form broth microdilution (BMD) as the reference method. The MIC(50/90)s were as follows: BMD, 1/4 mu g/ml; Vitek2, 4/>= 8 mu g/ml; Etest, 2/4 mu g/ml; MTS, 0.5/2 mu g/ml. Vitek2 produced 9.1/21.2% major errors, Etest produced 0.4/0.8% major errors, and MTS produced no major errors but 0.4/3.3% very major errors (FDA/EUCAST breakpoints). Vitek2 tigecycline results require confirmation by BMD or Etest for multidrug-resistant pathogens.