| dc.creator | Papagiannitsis, C. C. | en |
| dc.creator | Studentova, V. | en |
| dc.creator | Izdebski, R. | en |
| dc.creator | Oikonomou, O. | en |
| dc.creator | Pfeifer, Y. | en |
| dc.creator | Petinaki, E. | en |
| dc.creator | Hrabak, J. | en |
| dc.date.accessioned | 2015-11-23T10:43:32Z | |
| dc.date.available | 2015-11-23T10:43:32Z | |
| dc.date.issued | 2015 | |
| dc.identifier | 10.1128/jcm.03094-14 | |
| dc.identifier.issn | 0095-1137 | |
| dc.identifier.uri | http://hdl.handle.net/11615/31789 | |
| dc.description.abstract | A comparison of a matrix-assisted laser desorption ionization-time of flight mass spectrometric (MALDI-TOF MS) meropenem hydrolysis assay with the Carba NP test showed that both methods exhibited low sensitivity (approximately 76%), mainly due to the false-negative results obtained with OXA-48-type producers. The addition of NH4HCO3 to the reaction buffer for the MALDI-TOF MS assay dramatically improved its sensitivity (98%). Automatic interpretation of the MALDI-TOF MS assay, using the MBT STAR-BL software, generally agreed with the results obtained after manual analysis. For the Carba NP test, spectrophotometric analysis found six additional carbapenemase producers. | en |
| dc.source.uri | <Go to ISI>://WOS:000353036500041 | |
| dc.subject | CARBA NP TEST | en |
| dc.subject | BETA-LACTAMASE | en |
| dc.subject | RAPID DETECTION | en |
| dc.subject | KLEBSIELLA-PNEUMONIAE | en |
| dc.subject | RESISTANT ENTEROBACTERIACEAE | en |
| dc.subject | PHENOTYPIC DETECTION | en |
| dc.subject | PSEUDOMONAS | en |
| dc.subject | HOSPITALS | en |
| dc.subject | EMERGENCE | en |
| dc.subject | SPREAD | en |
| dc.subject | Microbiology | en |
| dc.title | Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Meropenem Hydrolysis Assay with NH4HCO3, a Reliable Tool for Direct Detection of Carbapenemase Activity | en |
| dc.type | journalArticle | en |
