Finding and analyzing plant metabolic gene clusters
Datum
2012Schlagwort
Zusammenfassung
Plants produce an array of diverse secondary metabolites with important ecological functions, providing protection against pests, diseases, and abiotic stresses. Secondary metabolites are also a rich source of bioactive compounds for drug and agrochemical development. Despite the importance of these compounds, the metabolic diversity of plants remains largely unexploited, primarily due to the problems associated with mining large and complex genomes. It has recently emerged that genes for the synthesis of multiple major classes of plant-derived secondary metabolites (benzoxinones, diterpenes, triterpenes, and cyanogenic glycosides) are organized in clusters reminiscent of the metabolic gene clusters found in microbes. Many more secondary metabolic clusters are likely to emerge as the body of sequence information available for plants continues to grow, accelerated by high-throughput sequencing. Here, we describe approaches for the identification of secondary metabolic gene clusters in plants through forward and reverse genetics, map-based cloning, and genome mining and give examples of methods used for the analysis and functional confirmation of new clusters. © 2012 Elsevier Inc. All rights reserved.
Collections
Verwandte Dokumente
Anzeige der Dokumente mit ähnlichem Titel, Autor, Urheber und Thema.
-
Lotus SHAGGY-like kinase 1 is required to suppress nodulation in Lotus japonicus
Garagounis C., Tsikou D., Plitsi P.K., Psarrakou I.S., Avramidou M., Stedel C., Anagnostou M., Georgopoulou M.E., Papadopoulou K.K. (2019)Glycogen synthase kinase/SHAGGY-like kinases (SKs) are a highly conserved family of signaling proteins that participate in many developmental, cell-differentiation, and metabolic signaling pathways in plants and animals. ... -
Cytological and other aspects of pathogenesis-related gene expression in tomato plants grown on a suppressive compost
Kavroulakis, N.; Papadopoulou, K. K.; Ntougias, S.; Zervakis, G. I.; Ehaliotis, C. (2006)• Background and Aims: Recent studies have shown that certain composts may trigger indirect defence mechanisms by sensitizing the plant to create an increased state of resistance, similar to systemic acquired resistance. ... -
Overexpression of a biotic stress‐inducible pvgstu gene activates early protective responses in tobacco under combined heat and drought
Stavridou E., Voulgari G., Michailidis M., Kostas S., Chronopoulou E.G., Labrou N.E., Madesis P., Nianiou‐obeidat I. (2021)Drought and heat stresses are major factors limiting crop growth and productivity, and their effect is more devastating when occurring concurrently. Plant glutathione transferases (GSTs) are differentially expressed in ...