| dc.description.abstract | This study aimed to investigate whether challenge with storage mites elicited flare ups of atopic dermatitis (AD) in Dermatophagoides farinae sensitized atopic Beagles housed in a house dust and storage mite-free environment. Atopic Beagles were environmentally challenged with 50 mg of Tyrophagus putrescentiae for three days in a row. Clinical signs were scored before, 6 h after each challenge and then every 24 h for a total of 5 days using a Canine Atopic Dermatitis Extent and Severity Index. Four healthy Beagles, negative on serology and intradermal testing for both house dust and storage mites, were used as controls and similarly challenged. A month after environmental challenge, the atopic Beagles were challenged by the oral route (50 mg of T. putrescentiae for three days in a row) and evaluated as described. Analyses of variance (ANOVAs) were used for comparisons between groups and types of challenges. All atopic Beagles developed erythematous pruritic lesions clinically compatible with AD on the face, pinnae, feet and ventral abdomen after both environmental and oral challenge. Control dogs did not develop dermatitis except for mild pinnal erythema in one dog. In the environmental challenge, ANOVA showed a significant effect of time, group, and group x time interaction, with atopic Beagles showing significantly higher scores than the controls. There were no significant differences in clinical scores after oral and environmental challenge in the atopic group. Cross-reactivity between house dust and storage mites could therefore contribute to flare ups of AD in house dust mite allergic dogs. | en |
