Polyphenolic composition of grape stem extracts affects antioxidant activity in endothelial and muscle cells
Auteur
Goutzourelas, N.; Stagos, D.; Spanidis, Y.; Liosi, M.; Apostolou, A.; Priftis, A.; Haroutounian, S.; Spandidos, D. A.; Tsatsakis, A. M.; Kouretas, D.Date
2015Sujet
Résumé
The aim of the present study was the assessment of the antioxidant effects of polyphenolic extracts derived from the stems of three Greek grape varieties (Moshomayro, Mavrotragano and Mandilaria) in endothelial (EA.hy926) and muscle (C2C12) cells. We also investigated the effects of the polyphenolic composition on the antioxidant effects of the grape stem extracts. For this purpose, the endothelial and muscle cells were treated with low non-cytotoxic concentrations of the extracts for 24 h in order to assess the effects of the extracts on cellular redox status using oxidative stress biomarkers. The oxidative stress markers were thiobarbituric acid reactive substances (TBARS), protein carbonyl (CARB) levels, reactive oxygen species (ROS) levels and glutathione (GSH) levels. The results revealed that treatment of the EA.hy926 cells with.
Collections
Related items
Showing items related by title, author, creator and subject.
-
Halophytic herbs of the Mediterranean basin: An alternative approach to health
Petropoulos S.A., Karkanis A., Martins N., Ferreira I.C.F.R. (2018)Wild native species are usually grown under severe and stressful conditions, while a special category includes halophytic species that are tolerant to high salinity levels. Native halophytes are valuable sources of bioactive ... -
Extracts from the mediterranean food plants Carthamus lanatus, Cichorium intybus, and Cichorium spinosum enhanced GSH levels and increased Nrf2 expression in human endothelial cells
Stagos D., Balabanos D., Savva S., Skaperda Z., Priftis A., Kerasioti E., Mikropoulou E.V., Vougogiannopoulou K., Mitakou S., Halabalaki M., Kouretas D. (2018)The Mediterranean diet is considered to prevent several diseases. In the present study, the antioxidant properties of six extracts from Mediterranean plant foods were assessed. The extracts' chemical composition analysis ... -
Investigation of Extraction Methods for the Assessment of the Pseudo-Total Concentration of Potentially Toxic Elements in Moderately Contaminated Soils of Central Greece
Golia E.E., Tsiropoulos N.G., Vleioras S., Antoniadis V. (2020)The aim of this research was the investigation of the effectiveness of several extractants as possible assessment of pseudo-total As, Pb and Cu concentrations in moderately contaminated soils of Central Greece. In soils ...