Effect of single dose irradiation on human glioblastoma spheroids in vitro
Auteur
Fehlauer, F.; Stalpers, L. J. A.; Panayiotides, J.; Kaaijk, P.; González Gonźalez, D.; Leenstra, S.; Van Der Valk, P.; Sminia, P.Date
2004Sujet
Résumé
Investigation of the predictive value of a radiosurgery-relevant treatment of glioblastoma spheroids. Organotypic multicellular spheroids were cultured and irradiated (20 Gy). Morphology, apoptosis and immunohistochemical expression of p53, p21, MIB-1, TGF-β and VEGF were examined 4 h, 24 h, 7 days, and 14 days following treatment. Cell proliferation decreased, while apoptosis was increased. No morphological damage was observed. p53 expression was significantly increased after 4 h. TGF-β and VEGF expression were only slightly altered. Particularly early changes in proliferation and apoptosis can be observed in spheroids. Individual response differences suggest spheroids of human gliomas to be useful for monitoring radiosurgery effects.