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Urate crystals induce NLRP3 inflammasome-dependent IL-1 beta secretion and proliferation in isolated primary human T-cells
dc.creator | Eleftheriadis, T. | en |
dc.creator | Pissas, G. | en |
dc.creator | Antoniadi, G. | en |
dc.creator | Makri, P. | en |
dc.creator | Liakopoulos, V. | en |
dc.creator | Stefanidis, I. | en |
dc.date.accessioned | 2015-11-23T10:26:12Z | |
dc.date.available | 2015-11-23T10:26:12Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1108-4189 | |
dc.identifier.uri | http://hdl.handle.net/11615/27333 | |
dc.description.abstract | Background: Urate through NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome-dependent caspase-1 activation stimulates macrophages to secrete inteleukin-1 beta (IL-1 beta). Urate also enhances adaptive immunity indirectly through its effect on antigen presenting cells. In this study, the direct effect of urate on isolated primary human T-cells was evaluated. Methods: Isolated T-cells were cultured with or without monosodium urate crystals in the presence or not of the NLRP3 inflammasome inhibitor glyburide. Activated cleaved caspase-1 was assessed by means of western blotting, whereas caspase-1 activity was measured colorimetrically in the cell lysates. IL-1 beta was measured in the supernatants by means of enzyme-linked immunosorbent assay. T-cell proliferation was assessed by means of bromodeoxyuridine labelling and immunoenzymatic detection. Results: Urate induced caspase-1 activation and IL-1 beta release by T-cells. It also induced proliferation of T-cells. Glyburide inhibited urate-induced caspase-1 activation, IL-1 beta secretion and proliferation. Conclusions: Urate, a well defined danger signal, stimulates directly human T-cells in a NLRP3 infmmasomela-dependent way. The subsequent IL-1 beta secretion could enhance inflammation, whereas expansion of T-cell clones could facilitate a subsequent adaptive immune response. | en |
dc.source | Hippokratia | en |
dc.source.uri | <Go to ISI>://WOS:000356628700008 | |
dc.subject | Urate | en |
dc.subject | T-cells | en |
dc.subject | caspase-1 | en |
dc.subject | interleukin-1 beta | en |
dc.subject | NLRP3 | en |
dc.subject | inflammasome | en |
dc.subject | URIC-ACID PROMOTES | en |
dc.subject | NALP3 INFLAMMASOME | en |
dc.subject | DENDRITIC CELLS | en |
dc.subject | DANGER SIGNAL | en |
dc.subject | ACTIVATION | en |
dc.subject | LYMPHOCYTES | en |
dc.subject | BINDING | en |
dc.subject | SILICA | en |
dc.subject | MICE | en |
dc.subject | ATP | en |
dc.subject | Medicine, General & Internal | en |
dc.title | Urate crystals induce NLRP3 inflammasome-dependent IL-1 beta secretion and proliferation in isolated primary human T-cells | en |
dc.type | journalArticle | en |
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