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Non-contact Current Transfer Induces the Formation and Improves the X-ray Diffraction Quality of Protein Crystals
dc.creator | Boltsis, I. | en |
dc.creator | Lagoumintzis, G. | en |
dc.creator | Chatzileontiadou, D. S. M. | en |
dc.creator | Giastas, P. | en |
dc.creator | Tzartos, S. J. | en |
dc.creator | Leonidas, D. D. | en |
dc.creator | Poulas, K. | en |
dc.date.accessioned | 2015-11-23T10:23:59Z | |
dc.date.available | 2015-11-23T10:23:59Z | |
dc.date.issued | 2014 | |
dc.identifier | 10.1021/cg5004098 | |
dc.identifier.issn | 1528-7483 | |
dc.identifier.uri | http://hdl.handle.net/11615/26346 | |
dc.description.abstract | The effect of non-contact current transfer on the crystallization of three model proteins (hen egg white lysozyme, bovine pancreatic ribonuclease A, and human recombinant insulin) was investigated using a prototype ion-generator device. The device can produce an equivalent of microcurrent that can be transmitted in a contactless manner by spraying negatively charged ions and using available air gases (O-2) as a transfer medium. From a crystallographic perspective, non-contact current transfer technology clearly enhanced the crystal formation process of these three proteins, as evidenced by the high crystallization speed and crystal quality. The quality and X-ray diffraction patterns of lysozyme crystals grown in the presence and absence of the device's microcurrent were analyzed. Our results indicate a significant positive effect on the quality of the X-ray diffraction pattern after non-contact current transfer, highlighting the value of this technique in protein crystallization. | en |
dc.source | Crystal Growth & Design | en |
dc.source.uri | <Go to ISI>://WOS:000341226500018 | |
dc.subject | EXTERNAL ELECTRIC-FIELD | en |
dc.subject | CRYSTALLIZATION | en |
dc.subject | NUCLEATION | en |
dc.subject | INSULIN | en |
dc.subject | Chemistry, Multidisciplinary | en |
dc.subject | Crystallography | en |
dc.subject | Materials Science, | en |
dc.subject | Multidisciplinary | en |
dc.title | Non-contact Current Transfer Induces the Formation and Improves the X-ray Diffraction Quality of Protein Crystals | en |
dc.type | journalArticle | en |
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