Auflistung Nach Schlagwort "POLYMERASE CHAIN-REACTION"
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Application of random amplified polymorphic DNA (RAPD) markers to evaluate intraspecific genetic variation in red mullet (Mullus barbatus)
(1998)The random amplified polymorphic DNA (RAPD) technique was used to evaluate genetic affinities among eight red mullet (Mullus barbatus L., 1758) samples from the Mediterranean Sea. Twenty-nine random primers were used. ... -
A comparative study of immunocapture ELISA and RT-PCR for screening clinical samples from Southern Greece for human influenza virus types A and B
(2000)An immunocapture (IC) ELISA and reverse transcriptase (RT)-PCR assays were evaluated as screening methods for the detection of influenza virus types A and B in clinical samples collected from individuals presenting with ... -
Comparison of Greek breeds of horses using RAPD markers
(2001)To assess genetic markers able to distinguish between five Greek breeds of horses, the random amplified polymorphic DNA (RAPD) technique was used. A total of 40 primers were initially screened in all breeds to identify ... -
Detection and characterization of phytoplasmas infecting tomato plants in Greece
(2007)Over the course of the 2005 and 2006 growing seasons, surveys were carried out in tomato fields in different parts of Greece in order to determine the occurrence and distribution of phytoplasma diseases of tomato, and to ... -
High sequence divergence in the 5 ' non-coding region of reference Coxsackie B and ECHO viral strains and clinical isolates revealed by restriction fragment length polymorphism analysis
(2001)We report the restriction fragment length polymorphism (RFLP) patterns of a 440-bp-long 5' non-coding region (5'NCR) amplification target of all 34 reference Coxsackie B and ECHO (enteric cytopathic human orphan) enterovirus ... -
Identification of staphylococci by Phoenix: validation of a new protocol and comparison with Vitek 2
(2010)Because of their frequent isolation in the routine laboratory and their increasing clinical significance, fast and accurate species identification of staphylococci may be required, this can only be achieved by automated ... -
Improved genotyping vaccine and wild-type poliovirus strains by restriction fragment length polymorphism analysis: Clinical diagnostic implications
(2000)The combination of preventive vaccination and diagnostic typing of viral isolates from patients with clinical poliomyelitis constitutes our main protective shield against polioviruses. The restriction fragment length ... -
Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities
(2007)The study demonstrates the presence of a nodavirus that affected sturgeon in fresh water, causing disease with neurological signs. The virus was isolated and inoculated onto SSN-1 (striped snakehead, Channa striatus) cell ... -
A modified method for the detection of differentially expressed mRNAs without using radioactivity
(2001)We present here a modification of the original differential display approach using a single oligo(dT) primer for the reverse transcription reaction (instead of the various oligo(dT)NM primers that subdivide the pool of ... -
Two successfully treated cases of Staphylococcus lugdunensis endocarditis
(2010)Prosthetic valve and pacemaker lead endocarditis by Staphylococcus lugdunensis remain very rare, while the former is associated with an ominous prognosis Two cases involving a prosthetic aortic valve and a pacemaker lead, ... -
Value of anti-HBc screening of blood donors for prevention of HBV infection: results of a 3-year prospective study in Northwestern Greece
(2001)BACKGROUND: The risk of infection with transfusion-transmitted viruses has been reduced remarkably. However, a zero-risk blood supply remains a popular goal. Some authorities have introduced the screening for antibody to ...