Parcourir par auteur "Ruether, I. G. A."
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Characterization of novel intergenogroup and intergenotype recombinant noroviruses from central Greece
Ruether, I. G. A.; Dimitriou, T. G.; Tsakogiannis, D.; Kyriakopoulou, Z.; Amoutzias, G. D.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2014)Noroviruses (NoVs) are a major causative agent of acute gastroenteritis in humans. They are members of the Caliciviridae family and based on the genetic analysis of the RdRp and capsid regions, human NoVs are divided into ... -
Circulation of intergenotype recombinant noroviruses GII.9/GII.6 from 2006 to 2011 in central Greece
Ruether, I. G. A.; Tsakogiannis, D.; Kyriakopoulou, Z.; Dimitriou, T. G.; Papamichail, C.; Gartzonika, C.; Leveidiotou-Stefanou, S.; Markoulatos, P. (2014)Noroviruses (NoVs) are members of the Caliciviridae family and are recognized as a worldwide cause of acute nonbacterial gastroenteritis. Based on the genetic analysis of the RdRp and capsid regions, human NoVs are divided ... -
Combined 5 ' UTR RFLP analysis and VP1 sequencing for epidemic investigation of enteroviruses
Kyriakopoulou, Z.; Tsolis, K.; Pliaka, V.; Tsakogiannis, D.; Ruether, I. G. A.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2013)Enteroviruses, the main cause of aseptic meningitis, consist of 100 serotypes, and many of them have been associated with large outbreaks. In the present study, a comparison of RFLP analysis of the 5' untranslated region ... -
Complete nucleotide sequence analysis of the VP1 genomic region of Echoviruses 6 isolated from sewage in Greece revealed 98% similarity with Echoviruses 6 that were characterized from an aseptic meningitis outbreak 1 year later
Kyriakopoulou, Z.; Dedepsidis, E.; Pliaka, V.; Tsakogiannis, D.; Ruether, I. G. A.; Krikelis, A.; Markoulatos, P. (2011)The molecular characterization of two enterovirus strains (LR51A5 and LR61G3) isolated from the sewage treatment plant unit in Larissa, Greece, in May and June 2006 and the investigation of their relationship with enteroviruses ... -
Correlation of mutations and recombination with growth kinetics of poliovirus vaccine strains
Pliaka, V.; Kyriakopoulou, Z.; Tsakogiannis, D.; Ruether, I. G. A.; Gartzonika, C.; Levidiotou-Stefanou, S.; Krikelis, A.; Markoulatos, P. (2010)Attenuated strains of Sabin poliovirus vaccine replicate in the human gut and, in rare cases, may cause vaccine-associated paralytic poliomyelitis (VAPP). The genetic instability of Sabin strains constitutes one of the ... -
Determination of antigenic properties of vaccine derived poliovirus strains
Pliaka, V.; Achilleos, C.; Kyriakopoulou, Z.; Tsakogiannis, D.; Ruether, I. G. A.; Gartzonica, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2010)In this study, the immunity level of the southern Greek population in the 1-10-year, 11-20-year, 21-30-year and 31-40-year age groups with regard to Sabin vaccine strains and a collection of 11 recombinant and three ... -
Determination of human papillomavirus 16 physical status through E1/E6 and E2/E6 ratio analysis
Tsakogiannis, D.; Kyriakopoulou, Z.; Ruether, I. G. A.; Amoutzias, G. D.; Dimitriou, T. G.; Diamantidou, V.; Kotsovassilis, C.; Markoulatos, P. (2014)Human papillomavirus (HPV) 16 genome integration into the host chromosome is a crucial event during the life cycle of the virus and a major step towards carcinogenesis. The integration of HPV16 DNA promotes a constitutive ... -
Duplex Real-time PCR assay and SYBR green I melting curve analysis for molecular identification of HPV genotypes 16, 18, 31, 35, 51 and 66
Tsakogiannis, D.; Papacharalampous, M.; Toska, E.; Kyriakopoulou, Z.; Dimitriou, T. G.; Ruether, I. G. A.; Komiotis, D.; Markoulatos, P. (2015)Long-term infection with high-risk HPV genotypes is the leading cause of cervical cancer. In the present study a Duplex Real-time PCR assay was developed in order to identify HPV types 16, 18, 31, 35, 51 and 66 in three ... -
Genome analysis of two type 6 echovirus (E6) strains recovered from sewage specimens in Greece in 2006
Kyriakopoulou, Z.; Pliaka, V.; Tsakogiannis, D.; Ruether, I. G. A.; Komiotis, D.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2012)Echovirus 6 (E6) is one of the main enteroviral serotypes that was isolated from cases of aseptic meningitis and encephalitis during the last years in Greece. Two E6 (LR51A5 and LR61G3) were isolated from the sewage treatment ... -
Identification of novel E6-E7 sequence variants of human papillomavirus 16
Tsakogiannis, D.; Kyriakopoulou, Z.; Amoutzias, G.; Ruether, I. G. A.; Dimitriou, T. G.; Panotopoulou, E.; Markoulatos, P. (2013)The rate of evolution of the human papillomavirus 16 (HPV16) genome is low. However, the ability of the E6 oncoprotein to interact with distinct p53 variants causes selective pressure on the E6 gene. In addition, intratypic ... -
Molecular and evolutionary analysis of HPV16 E6 and E7 genes in Greek women
Tsakogiannis, D.; Papadopoulou, A.; Kontostathi, G.; Ruether, I. G. A.; Kyriakopoulou, Z.; Dimitriou, T. G.; Orfanoudakis, G.; Markoulatos, P. (2013)Human papillomavirus type 16 (HPV16) non-European variants have been associated with persistent infection and cervical cancer development, while the L83V variant of the E6 gene has been correlated with the progression of ... -
Molecular and phylogenetic analysis of the HPV 16 E4 gene in cervical lesions from women in Greece
Tsakogiannis, D.; Ruether, I. G. A.; Kyriakopoulou, Z.; Pliaka, V.; Skordas, V.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2012)The HPV16 E1(a )E4 protein is thought to contribute to the release of newly formed viral particles from infected epithelia. In order to investigate amino acid mutations in the HPV16 E1(a )E4 protein, the complete E4 ORF ... -
Molecular characterization of a new intergenotype Norovirus GII recombinant
Ruether, I. G. A.; Tsakogiannis, D.; Pliaka, V.; Kyriakopoulou, Z.; Krikelis, A.; Gartzonika, C.; Leveidiotou-Stefanou, S.; Markoulatos, P. (2012)Human noroviruses (NoVs) of the Caliciviridae family are a major cause of epidemic gastroenteritis. The NoV genus is genetically diverse and recombination of viral RNA is known to depend upon various immunological and ... -
Nucleotide polymorphisms of the human papillomavirus 16 E1 gene
Tsakogiannis, D.; Darmis, F.; Gortsilas, P.; Ruether, I. G. A.; Kyriakopoulou, Z.; Dimitriou, T. G.; Amoutzias, G.; Markoulatos, P. (2014)The E1 ORF is one of the most conserved regions in the human papillomavirus (HPV) genome. The complete E1 gene of the HPV16 genome was amplified with four overlapping primer sets in 16 high-grade (CIN II, III) and 13 ... -
PCR assays for the identification of rare recombination types from VP1 to 3D genomic region of vaccine derived poliovirus strains
Dimitriou, T. G.; Pliaka, V.; Kyriakopoulou, Z.; Ruether, I. G. A.; Tsakogiannis, D.; Fountoucidou, P.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2014)Poliomyelitis has been effectively controlled by the use of inactivated poliovirus vaccine (IPV) or trivalent live attenuated oral poliovirus vaccine (OPV). Since 1964, the use of OPV in mass vaccinations has resulted in ... -
Prevalence of HPV16 E1-1374 boolean AND 63nt variants in Greek women
Tsakogiannis, D.; Kyriakopoulou, Z.; Darmis, F.; Ruether, I. G. A.; Dimitriou, T. G.; Orfanoudakis, G.; Panotopoulou, E.; Markoulatos, P. (2014)Recent studies have focused on sequence variation of the HPV16 E1 gene. The present study investigates the prevalence of E1-1374<^>63nt duplication in the Greek population, and the sequence variation at the 5 ' end of the ... -
Retrospective molecular and phenotypic analysis of poliovirus vaccine strains isolated in Greece
Pliaka, V.; Filliponi, M. E.; Kyriakopoulou, Z.; Ruether, I. G. A.; Tsakogiannis, D.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2011)The live oral poliovirus vaccine (OPV) strains are genetically unstable, causing, in rare cases, vaccine-associated paralytic poliomyelitis. Reversions of the known attenuating mutations in OPV strains and intertypic ... -
Sequence variation analysis of the E2 gene of human papilloma virus type 16 in cervical lesions from women in Greece
Tsakogiannis, D.; Ruether, I. G. A.; Kyriakopoulou, Z.; Pliaka, V.; Theoharopoulou, A.; Skordas, V.; Panotopoulou, E.; Nepka, C.; Markoulatos, P. (2012)The E2 gene of human papilloma virus is expressed at the early stage of the viral life cycle, encoding the E2 transcription factor, and regulates the expression of E6 and E7 oncogenes. Disruption of E2 gene due to viral ... -
A seroprevalence study of poliovirus antibody against a collection of recombinant and non-recombinant poliovirus vaccine strains in the population of southern Greece
Pliaka, V.; Ruether, I. G. A.; Kyriakopoulou, Z.; Kioussi, P.; Plakokefalos, E.; Megalou, M.; Pratti, A.; Levidiotou-Stefanou, S.; Markoulatos, P. (2010)P>In this study, the serological status of the southern Greek population in the 1-10-year, 11-20-year, 21-30-year and 31-40 -year age groups with regard to Sabin vaccine strains and a collection of 15 recombinant and four ... -
Sites of disruption within E1 and E2 genes of HPV16 and association with cervical dysplasia
Tsakogiannis, D.; Gortsilas, P.; Kyriakopoulou, Z.; Ruether, I. G. A.; Dimitriou, T. G.; Orfanoudakis, G.; Markoulatos, P. (2015)Integration of HPV16 DNA into the host chromosome usually disrupts the E1 and/or E2 genes. The present study investigated the disruption of E1, E2 genes in a total of eighty four HPV16-positive precancerous and cervical ...