Browsing by Subject "amino acid sequence"

The 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6’-methylresorufin) ester (DGGR) lipase assay in cats and dogs is not specific for pancreatic lipase

Lim S.Y., Xenoulis P.G., Stavroulaki E.M., Lidbury J.A., Suchodolski J.S., Carrière F., Steiner J.M. (2020)

Background: The measurement of pancreatic lipase is important for the diagnosis of feline and canine pancreatitis. Recent studies have claimed that lipase assays using the 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6’-m ...

Achilles, a new family of transcriptionally active retrotransposons from the olive fruit fly, with y chromosome preferential distribution

Tsoumani K.T., Drosopoulou E., Bourtzis K., Gariou-Papalexiou A., Mavragani-Tsipidou P., Zacharopoulou A., Mathiopoulos K.D. (2015)

Sex chromosomes have many unusual features relative to autosomes. The in depth exploration of their structure will improve our understanding of their origin and divergence (degeneration) as well as the evolution of genetic ...

Amino acid signatures in the HLA class II peptide-binding region associated with protection/susceptibility to the severe West Nile Virus disease

Sarri C.A., Papadopoulos G.E., Papa A., Tsakris A., Pervanidou D., Baka A., Politis C., Billinis C., Hadjichristodoulou C., Mamuris Z. (2018)

The MHC class II region in humans is highly polymorphic. Each MHC molecule is formed by an α and a β chain, produced by different genes, creating an antigen-binding groove. In the groove there are several pockets into which ...

Ancestral Absence of Electron Transport Chains in Patescibacteria and DPANN

Beam J.P., Becraft E.D., Brown J.M., Schulz F., Jarett J.K., Bezuidt O., Poulton N.J., Clark K., Dunfield P.F., Ravin N.V., Spear J.R., Hedlund B.P., Kormas K.A., Sievert S.M., Elshahed M.S., Barton H.A., Stott M.B., Eisen J.A., Moser D.P., Onstott T.C., Woyke T., Stepanauskas R. (2020)

Recent discoveries suggest that the candidate superphyla Patescibacteria and DPANN constitute a large fraction of the phylogenetic diversity of Bacteria and Archaea. Their small genomes and limited coding potential have ...

Anosmic flies: what Orco silencing does to olive fruit flies

Tsoumani K.T., Belavilas-Trovas A., Gregoriou M.-E., Mathiopoulos K.D. (2020)

Background: The olive fruit fly (Bactrocera oleae) is the most destructive pest of the olive cultivation worldwide causing significant production losses and olive fruit impoverishment, as its larvae feed exclusively on the ...

AtHESPERIN: A novel regulator of circadian rhythms with poly(A)-degrading activity in plants

Delis C., Krokida A., Tomatsidou A., Tsikou D., Beta R.A.A., Tsioumpekou M., Moustaka J., Stravodimos G., Leonidas D.D., Balatsos N.A.A., Papadopoulou K.K. (2016)

We report the identification and characterization of a novel gene, AtHesperin (AtHESP) that codes for a deadenylase in Arabidopsis thaliana. The gene is under circadian clock-gene regulation and has similarity to the ...

Biochemical and genetic evidence for the presence of multiple phosphatidylinositol- and phosphatidylinositol 4,5-bisphosphate-specific phospholipases C in Tetrahymena

Leondaritis, G.; Sarri, T.; Dafnis, I.; Efstathiou, A.; Galanopoulou, D. (2011)

Eukaryotic phosphoinositide-specific phospholipases C (PI-PLC) specifically hydrolyze phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2], produce the Ca2+-mobilizing agent inositol 1,4,5-trisphosphate, and regulate ...

Comparative Analysis of SARS-CoV-2 Variants of Concern, Including Omicron, Highlights Their Common and Distinctive Amino Acid Substitution Patterns, Especially at the Spike ORF

Nikolaidis M., Papakyriakou A., Chlichlia K., Markoulatos P., Oliver S.G., Amoutzias G.D. (2022)

In order to gain a deeper understanding of the recently emerged and highly divergent Omicron variant of concern (VoC), a study of amino acid substitution (AAS) patterns was performed and compared with those of the other ...

The complex evolutionary history of aminoacyl-tRNA synthetases

Chaliotis A., Vlastaridis P., Mossialos D., Ibba M., Becker H.D., Stathopoulos C., Amoutzias G.D. (2017)

Aminoacyl-tRNA synthetases (AARSs) are a superfamily of enzymes responsible for the faithful translation of the genetic code and have lately become a prominent target for synthetic biologists. Our largescale analysis of > ...

Conformational properties of HIV-1 gp120/V3 immunogenic domains

Galanakis, P. A.; Spyroulias, G. A.; Rizos, A.; Samolis, P.; Krambovitis, E. (2005)

Infection of target host cells by the human immunodeficiency virus-1 (HIV-1) is a multi-step process involving a series of conformational changes in the viral gp120 and gp41 proteins. Gp120 binding to the main cell receptor, ...

Detection of unusual mutation within the VP1 region of different re-isolates of poliovirus Sabin vaccine

Dedepsidis, E.; Karakasiliotis, I.; Paximadi, E.; Kyriakopoulou, Z.; Komiotis, D.; Markoulatos, P. (2006)

In the present study, a genomic analysis of full VP1 sequence region of 15 clinical re-isolates (14 healthy vaccinees and one bone marrow tumor patient) was conducted, aiming to the identification of mutations and to the ...

The Drosophila septate junctions beyond barrier function: Review of the literature, prediction of human orthologs of the SJ-related proteins and identification of protein domain families

Rouka E., Gourgoulianni N., Lüpold S., Hatzoglou C., Gourgoulianis K., Blanckenhorn W.U., Zarogiannis S.G. (2021)

The involvement of Septate Junctions (SJs) in critical cellular functions that extend beyond their role as diffusion barriers in the epithelia and the nervous system has made the fruit fly an ideal model for the study of ...

Evidence for disulfide bonds in SR Protein Kinase 1 (SRPK1) that are required for activity and nuclear localization

Koutroumani M., Papadopoulos G.E., Vlassi M., Nikolakaki E., Giannakouros T. (2017)

Serine/arginine protein kinases (SRPKs) phosphorylate Arg/Ser dipeptide-containing proteins that play crucial roles in a broad spectrum of basic cellular processes. The existence of a large internal spacer sequence that ...

Frequency dependent non-thermal effects of oscillating electric fields in the microwave region on the properties of a solvated lysozyme system: A molecular dynamics study

Floros S., Liakopoulou-Kyriakides M., Karatasos K., Papadopoulos G.E. (2017)

The use of microwaves in every day's applications raises issues regarding the non thermal biological effects of microwaves. In this work we employ molecular dynamics simulations to advance further the dielectric studies ...

Hidden neural networks for transmembrane protein topology prediction

Tamposis I.A., Sarantopoulou D., Theodoropoulou M.C., Stasi E.A., Kontou P.I., Tsirigos K.D., Bagos P.G. (2021)

Hidden Markov Models (HMMs) are amongst the most successful methods for predicting protein features in biological sequence analysis. However, there are biological problems where the Markovian assumption is not sufficient ...

HIF-1α-derived cell-penetrating peptides inhibit ERK-dependent activation of HIF-1 and trigger apoptosis of cancer cells under hypoxia

Karagiota A., Kourti M., Simos G., Mylonis I. (2019)

Hypoxia is frequently encountered in the microenvironment of solid tumors. Hypoxia-inducible factors (HIFs), the main effectors of cell response to hypoxia, promote cancer cell survival and progression. HIF-1α, the ...