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Identification of rearranged sequences of HPV16 DNA in precancerous and cervical cancer cases

dc.creatorTsakogiannis D., Bletsa M., Kyriakopoulou Z., Dimitriou T.G., Kotsovassilis C., Panotopoulou E., Markoulatos P.en
dc.date.accessioned2023-01-31T10:10:54Z
dc.date.available2023-01-31T10:10:54Z
dc.date.issued2016
dc.identifier10.1016/j.mcp.2015.11.004
dc.identifier.issn08908508
dc.identifier.urihttp://hdl.handle.net/11615/79819
dc.description.abstractIntegration of HPV16 DNA into the host chromosome is considered to be a crucial step towards genomic instability and cervical cancer development. Aim of the present study was to investigate the presence of HPV16 rearranged intra-viral sequences in HPV16-positive normal, precancerous and cervical cancer samples using the method of Restriction Site-PCR (RS-PCR). Sequence analysis of HPV16 integrants revealed for the first time in clinical samples two distinct rearranged intra-viral sequences, concerning the conjunction of E2 and L1 genes and the conjunction of E1 and L1 genes with inverted orientation. Furthermore mapping analysis of the E1 and E2 genes in cervical samples with rearranged intra-viral sequences of HPV16 genome was conducted in order to determine the integrity of viral genes. The identification of intra-viral rearrangements provides valuable information regarding the HPV16 DNA integration, and may be a significant biomarker for the presence of chromosomal instability and DNA damages in clinical samples. © 2015 Elsevier Ltd.en
dc.language.isoenen
dc.sourceMolecular and Cellular Probesen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84959330118&doi=10.1016%2fj.mcp.2015.11.004&partnerID=40&md5=b57efa107015b7f8c2c508fd4ddd42bd
dc.subjectglycoprotein E1en
dc.subjectglycoprotein E2en
dc.subjectvirus DNAen
dc.subjectvirus DNAen
dc.subjectArticleen
dc.subjectchromosomal instabilityen
dc.subjectDNA damageen
dc.subjectDNA integrationen
dc.subjectDNA sequenceen
dc.subjectfemaleen
dc.subjecthumanen
dc.subjectHuman papillomavirus type 16en
dc.subjecthuman tissueen
dc.subjectpolymerase chain reactionen
dc.subjectpriority journalen
dc.subjectsequence alignmenten
dc.subjectuterine cervix canceren
dc.subjectvirus genomeen
dc.subjectchemistryen
dc.subjectgene rearrangementen
dc.subjectgeneticsen
dc.subjectHuman papillomavirus type 16en
dc.subjectnucleotide sequenceen
dc.subjectpapillomavirus infectionen
dc.subjectprecanceren
dc.subjectproceduresen
dc.subjecttumor cell lineen
dc.subjectuterine cervix carcinoma in situen
dc.subjectuterine cervix tumoren
dc.subjectvirologyen
dc.subjectvirus geneen
dc.subjectBase Sequenceen
dc.subjectCell Line, Tumoren
dc.subjectCervical Intraepithelial Neoplasiaen
dc.subjectDNA, Viralen
dc.subjectFemaleen
dc.subjectGene Rearrangementen
dc.subjectGenes, Viralen
dc.subjectHuman papillomavirus 16en
dc.subjectHumansen
dc.subjectPapillomavirus Infectionsen
dc.subjectPolymerase Chain Reactionen
dc.subjectPrecancerous Conditionsen
dc.subjectSequence Analysis, DNAen
dc.subjectUterine Cervical Neoplasmsen
dc.subjectAcademic Pressen
dc.titleIdentification of rearranged sequences of HPV16 DNA in precancerous and cervical cancer casesen
dc.typejournalArticleen


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