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A low-protein, high-carbohydrate diet increases browning in perirenal adipose tissue but not in inguinal adipose tissue
dc.creator | Pereira M.P., Ferreira L.A.A., da Silva F.H.S., Christoffolete M.A., Metsios G.S., Chaves V.E., de França S.A., Damazo A.S., Flouris A.D., Kawashita N.H. | en |
dc.date.accessioned | 2023-01-31T09:47:16Z | |
dc.date.available | 2023-01-31T09:47:16Z | |
dc.date.issued | 2017 | |
dc.identifier | 10.1016/j.nut.2017.05.007 | |
dc.identifier.issn | 08999007 | |
dc.identifier.uri | http://hdl.handle.net/11615/78056 | |
dc.description.abstract | Objective The aim of this study was to evaluate the browning and origin of fatty acids (FAs) in the maintenance of triacylglycerol (TG) storage and/or as fuel for thermogenesis in perirenal adipose tissue (periWAT) and inguinal adipose tissue (ingWAT) of rats fed a low-protein, high-carbohydrate (LPHC) diet. Methods LPHC (6% protein, 74% carbohydrate) or control (C; 17% protein, 63% carbohydrate) diets were administered to rats for 15 d. The tissues were stained with hematoxylin and eosin for histologic analysis. The content of uncoupling protein 1 (UCP1) was determined by immunofluorescence. Levels of T-box transcription factor (TBX1), PR domain containing 16 (PRDM16), adipose triacylglycerol lipase (ATGL), hormone-sensitive lipase, lipoprotein lipase (LPL), glycerokinase, phosphoenolpyruvate carboxykinase (PEPCK), glucose transporter 4, β3-adrenergic receptor (AR), β1-AR, protein kinase A (PKA), adenosine-monophosphate-activated protein kinase (AMPK), and phospho-AMPK were determined by immunoblotting. Serum fibroblast growth factor 21 (FGF21) was measured using a commercial kit (Student's t tests, P < 0.05). Results The LPHC diet increased FGF21 levels by 150-fold. The presence of multilocular adipocytes, combined with the increased contents of UCP1, TBX1, and PRDM16 in periWAT of LPHC-fed rats, suggested the occurrence of browning. The contents of β1-AR and LPL were increased in the periWAT. The ingWAT showed higher ATGL and PEPCK levels, phospho-AMPK/AMPK ratio, and reduced β3-AR and PKA levels. Conclusion These findings suggest that browning occurred only in the periWAT and that higher utilization of FAs from blood lipoproteins acted as fuel for thermogenesis. Increased glycerol 3-phosphate generation by glyceroneogenesis increased FAs reesterification from lipolysis, explaining the increased TG storage in the ingWAT. © 2017 Elsevier Inc. | en |
dc.language.iso | en | en |
dc.source | Nutrition | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85025432516&doi=10.1016%2fj.nut.2017.05.007&partnerID=40&md5=cedfeb91426998833ae1c9281de9db9e | |
dc.subject | adenylate kinase | en |
dc.subject | beta 1 adrenergic receptor | en |
dc.subject | beta 3 adrenergic receptor | en |
dc.subject | carbohydrate | en |
dc.subject | cyclic AMP dependent protein kinase | en |
dc.subject | eosin | en |
dc.subject | fatty acid | en |
dc.subject | fibroblast growth factor 21 | en |
dc.subject | glucose transporter 4 | en |
dc.subject | glycerol kinase | en |
dc.subject | glycerophosphate | en |
dc.subject | hematoxylin | en |
dc.subject | hormone sensitive lipase | en |
dc.subject | hydroxymethylglutaryl coenzyme A reductase kinase | en |
dc.subject | lipoprotein lipase | en |
dc.subject | phosphoenolpyruvate carboxykinase (GTP) | en |
dc.subject | protein | en |
dc.subject | transcription factor | en |
dc.subject | transcription factor PRDM16 | en |
dc.subject | transcription factor TBX1 | en |
dc.subject | triacylglycerol | en |
dc.subject | triacylglycerol lipase | en |
dc.subject | unclassified drug | en |
dc.subject | uncoupling protein 1 | en |
dc.subject | fibroblast growth factor | en |
dc.subject | fibroblast growth factor 21 | en |
dc.subject | adipocyte | en |
dc.subject | animal cell | en |
dc.subject | animal experiment | en |
dc.subject | animal model | en |
dc.subject | animal tissue | en |
dc.subject | Article | en |
dc.subject | carbohydrate diet | en |
dc.subject | carbohydrate synthesis | en |
dc.subject | controlled study | en |
dc.subject | esterification | en |
dc.subject | fatty acid transport | en |
dc.subject | food composition | en |
dc.subject | glycation | en |
dc.subject | glyceroneogenesis | en |
dc.subject | histology | en |
dc.subject | immunoblotting | en |
dc.subject | immunofluorescence | en |
dc.subject | inguinal fat | en |
dc.subject | lipid storage | en |
dc.subject | lipolysis | en |
dc.subject | male | en |
dc.subject | nonhuman | en |
dc.subject | perirenal fat | en |
dc.subject | priority journal | en |
dc.subject | protein restriction | en |
dc.subject | rat | en |
dc.subject | thermogenesis | en |
dc.subject | white adipose tissue | en |
dc.subject | administration and dosage | en |
dc.subject | animal | en |
dc.subject | blood | en |
dc.subject | brown adipose tissue | en |
dc.subject | carbohydrate diet | en |
dc.subject | diet | en |
dc.subject | drug effects | en |
dc.subject | fluorescent antibody technique | en |
dc.subject | inguinal canal | en |
dc.subject | kidney | en |
dc.subject | metabolism | en |
dc.subject | procedures | en |
dc.subject | protein restriction | en |
dc.subject | Wistar rat | en |
dc.subject | Adipose Tissue, Brown | en |
dc.subject | Animals | en |
dc.subject | Diet | en |
dc.subject | Diet, Protein-Restricted | en |
dc.subject | Dietary Carbohydrates | en |
dc.subject | Fibroblast Growth Factors | en |
dc.subject | Fluorescent Antibody Technique | en |
dc.subject | Inguinal Canal | en |
dc.subject | Kidney | en |
dc.subject | Male | en |
dc.subject | Models, Animal | en |
dc.subject | Rats | en |
dc.subject | Rats, Wistar | en |
dc.subject | Elsevier Inc. | en |
dc.title | A low-protein, high-carbohydrate diet increases browning in perirenal adipose tissue but not in inguinal adipose tissue | en |
dc.type | journalArticle | en |
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