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Evidence of deregulated cholesterol efflux in abdominal aortic aneurysm
dc.creator | Mourmoura E., Vasilaki A., Giannoukas A., Michalodimitrakis E., Pavlidis P., Tsezou A. | en |
dc.date.accessioned | 2023-01-31T09:01:59Z | |
dc.date.available | 2023-01-31T09:01:59Z | |
dc.date.issued | 2016 | |
dc.identifier | 10.1016/j.acthis.2015.11.012 | |
dc.identifier.issn | 00651281 | |
dc.identifier.uri | http://hdl.handle.net/11615/76798 | |
dc.description.abstract | Previous studies indicated that lipids may be associated with abdominal aortic aneurysm (AAA); however the molecular mechanism involved is unclear. Our study aimed to investigate the expression pattern of cholesterol efflux related proteins in AAA. Liver X receptors (LXRα and LXRβ), ATP-binding-cassette transporter A1 (ABCA1), Apolipoprotein AI (ApoAI), smooth muscle α-actin (α-SM) and vimentin expression levels were evaluated in human AAA, atherosclerotic (ATH) and normal abdominal aortic tissues. We found significant differences in LXRα, LXRβ and ABCA1 mRNA expression levels between AAA, ATH and normal whole aortic tissues and also within the AAA, ATH and normal "intima-media" layers. Specifically, LXRα, LXRβ and ABCA1 mRNA levels were decreased in AAA compared to ATH-whole tissues, as well as in AAA "intima-media" compared to ATH and normal "intima-media" layers. Moreover, immunohistochemical evaluation revealed that LXRα and ABCA1 immunoreactivities (IR) were reduced in the AAA media compared to the normal and ATH media layers and that they were also reduced in the intima layer of AAA and ATH tissues, whereas ApoAI-IR was increased in the AAA and ATH aortic walls compared to normal pointing to possible deregulation of the cholesterol efflux mechanism in AAA. Furthermore, double staining for vimentin and α-SM showed vimentin expression in the intima and inner media layer of AAA with sparse vimentin positive SMCs designating possible SMCs phenotype switch from contractile to synthetic form. In addition, histochemical analysis showed excessive lipid accumulation in the AAA wall, while co-staining using Oil Red O with α-SM or CD68 revealed lipid accumulation in SMCs and macrophages, respectively. Our study provides novel evidence for impaired cholesterol efflux in AAA associated with lipid accumulation in SMCs and macrophages, as well as switch of SMCs phenotype from contractile to synthetic form. © 2015 Elsevier GmbH. | en |
dc.language.iso | en | en |
dc.source | Acta Histochemica | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84960155626&doi=10.1016%2fj.acthis.2015.11.012&partnerID=40&md5=9fe97af096236cd1d7f3abe4f5359b38 | |
dc.subject | ABC transporter A1 | en |
dc.subject | alpha smooth muscle actin | en |
dc.subject | apolipoprotein A1 | en |
dc.subject | carrier protein | en |
dc.subject | CD68 antigen | en |
dc.subject | cholesterol | en |
dc.subject | cholesterol efflux related protein | en |
dc.subject | liver X receptor alpha | en |
dc.subject | liver X receptor beta | en |
dc.subject | messenger RNA | en |
dc.subject | unclassified drug | en |
dc.subject | vimentin | en |
dc.subject | ABC transporter A1 | en |
dc.subject | ABCA1 protein, human | en |
dc.subject | cholesterol | en |
dc.subject | liver X receptor | en |
dc.subject | orphan nuclear receptor | en |
dc.subject | abdominal aorta aneurysm | en |
dc.subject | adult | en |
dc.subject | aged | en |
dc.subject | aorta atherosclerosis | en |
dc.subject | aorta intima | en |
dc.subject | aorta media | en |
dc.subject | aortic tissue | en |
dc.subject | Article | en |
dc.subject | cell maturation | en |
dc.subject | cholesterol transport | en |
dc.subject | clinical article | en |
dc.subject | controlled study | en |
dc.subject | disorders of lipid and lipoprotein metabolism | en |
dc.subject | female | en |
dc.subject | foam cell | en |
dc.subject | gene expression | en |
dc.subject | human | en |
dc.subject | human cell | en |
dc.subject | human tissue | en |
dc.subject | immunohistochemistry | en |
dc.subject | immunoreactivity | en |
dc.subject | lipid storage | en |
dc.subject | macrophage | en |
dc.subject | male | en |
dc.subject | pathogenesis | en |
dc.subject | phenotype | en |
dc.subject | protein expression | en |
dc.subject | smooth muscle fiber | en |
dc.subject | abdominal aorta | en |
dc.subject | abdominal aortic aneurysm | en |
dc.subject | atherosclerosis | en |
dc.subject | case control study | en |
dc.subject | cytoskeleton | en |
dc.subject | lipid metabolism | en |
dc.subject | metabolism | en |
dc.subject | middle aged | en |
dc.subject | pathology | en |
dc.subject | secretion (process) | en |
dc.subject | smooth muscle cell | en |
dc.subject | very elderly | en |
dc.subject | Aged | en |
dc.subject | Aged, 80 and over | en |
dc.subject | Aorta, Abdominal | en |
dc.subject | Aortic Aneurysm, Abdominal | en |
dc.subject | Atherosclerosis | en |
dc.subject | ATP Binding Cassette Transporter 1 | en |
dc.subject | Case-Control Studies | en |
dc.subject | Cholesterol | en |
dc.subject | Cytoskeleton | en |
dc.subject | Foam Cells | en |
dc.subject | Humans | en |
dc.subject | Lipid Metabolism | en |
dc.subject | Liver X Receptors | en |
dc.subject | Male | en |
dc.subject | Middle Aged | en |
dc.subject | Myocytes, Smooth Muscle | en |
dc.subject | Orphan Nuclear Receptors | en |
dc.subject | Elsevier GmbH | en |
dc.title | Evidence of deregulated cholesterol efflux in abdominal aortic aneurysm | en |
dc.type | journalArticle | en |
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