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HΜGB1/sRAGE levels differ significantly between transudates and exudates

dc.creatorKotsiou O.S., Jagirdar R.M., Papazoglou E.D., Hatzoglou C., Gourgoulianis K.I., Zarogiannis S.G.en
dc.date.accessioned2023-01-31T08:44:48Z
dc.date.available2023-01-31T08:44:48Z
dc.date.issued2021
dc.identifier10.1016/j.cyto.2021.155469
dc.identifier.issn10434666
dc.identifier.urihttp://hdl.handle.net/11615/75216
dc.description.abstractHigh mobility group box 1(HMGB1) protein operates as an alarmin with multiple roles in immunity and cell homeostasis. It is highly expressed in epithelial barrier sites and acts via the binding to the receptor for advanced glycation end products (RAGE). Production of HMGB1 and soluble RAGE (sRAGE), a decoy receptor for HMGB1, has been implicated in several pulmonary diseases, but both have been scarcely investigated in pleural diseases. The aim of this study was to determine the levels of HMGB1 and sRAGE in transudative, malignant and parapneumonic pleural effusions (PEs) and to investigate the effect of low and high HMGB1 pleural fluid levels on MeT-5A cell adhesion, migration and spheroid formation, in each group. HMGB1 and sRAGE levels were significantly lower and higher in transudative PEs compared to malignant and parapneumonic PEs, respectively. Patients above 65 years of age had significantly lower HMGB1 and higher sRAGE levels compared to patients below 65 years old. Furthermore, incubation of MeT-5A cells with malignant or parapneumonic PEs bearing low or high levels of HMGB1 yielded significant differential effects on MeT-5A cell adhesion, migration and spheroid formation. In all types of effusions, high HMGB1 levels correlated with more adherence compared to low HMGB1 levels. In transudative and malignant PEs high HMGB1 levels correlated with decreased migration of MeT-5A cells while in parapneumonic ones the effect was the opposite. Only samples from parapneumonic PEs high in HMGB1 achieved uniform spheroid formation. These results reveal a clinical context-dependent effect of the HMGB1/sRAGE axis in PEs. © 2021 Elsevier Ltden
dc.language.isoenen
dc.sourceCytokineen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85101006412&doi=10.1016%2fj.cyto.2021.155469&partnerID=40&md5=069799ced8ecc2934621fa3d21f40fa2
dc.subjectadvanced glycation end product receptoren
dc.subjecthigh mobility group B1 proteinen
dc.subjecthigh mobility group B1 proteinen
dc.subjectHMGB1 protein, humanen
dc.subjectmitogen activated protein kinaseen
dc.subjectMOK protein, humanen
dc.subjecttumor antigenen
dc.subjectage distributionen
dc.subjectageden
dc.subjectArticleen
dc.subjectcell adhesionen
dc.subjectcell migrationen
dc.subjectclinical articleen
dc.subjectcontrolled studyen
dc.subjectfemaleen
dc.subjecthumanen
dc.subjecthuman cellen
dc.subjectin vitro studyen
dc.subjectmaleen
dc.subjectmalignant pleura effusionen
dc.subjectmesothelium cellen
dc.subjectMeT-5A cell lineen
dc.subjectparapneumonic pleural effusionen
dc.subjectpleura effusionen
dc.subjectpleura fluiden
dc.subjectspheroid cellen
dc.subjecttransudative pleural effusionen
dc.subjectclinical trialen
dc.subjectexudateen
dc.subjectmetabolismen
dc.subjecttransformed cell lineen
dc.subjectAgeden
dc.subjectAntigens, Neoplasmen
dc.subjectCell Line, Transformeden
dc.subjectExudates and Transudatesen
dc.subjectFemaleen
dc.subjectHMGB1 Proteinen
dc.subjectHumansen
dc.subjectMaleen
dc.subjectMitogen-Activated Protein Kinasesen
dc.subjectPleural Effusion, Malignanten
dc.subjectAcademic Pressen
dc.titleHΜGB1/sRAGE levels differ significantly between transudates and exudatesen
dc.typejournalArticleen


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