dc.creator | Karagkouni D., Karavangeli A., Paraskevopoulou M.D., Hatzigeorgiou A.G. | en |
dc.date.accessioned | 2023-01-31T08:30:49Z | |
dc.date.available | 2023-01-31T08:30:49Z | |
dc.date.issued | 2021 | |
dc.identifier | 10.1007/978-1-0716-1697-0_21 | |
dc.identifier.issn | 10643745 | |
dc.identifier.uri | http://hdl.handle.net/11615/74356 | |
dc.description.abstract | Long noncoding RNAs (lncRNAs) are noncoding transcripts, usually longer than 200 nt, that constitute one of the largest and significantly heterogeneous RNA families. The annotation of lncRNAs and the characterization of their function is a constantly evolving field. LncRNA interplay with microRNAs (miRNAs) is thoroughly studied in several physiological and disease states. miRNAs are small noncoding RNAs (~22 nt) that posttranscriptionally regulate the expression of protein coding genes, through mRNA target cleavage, degradation or direct translational suppression. miRNAs can affect lncRNA half-life by promoting their degradation, or lncRNAs can act as miRNA “sponges,” reducing miRNA regulatory effect on target mRNAs. This chapter outlines the miRNA–lncRNA interplay and provides hands-on methodologies for experimentally supported and in silico-guided analyses. The proposed techniques are a valuable asset to further understand lncRNA functions and can be appropriately adapted to become the backbone for further downstream analyses. © 2021, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. | en |
dc.language.iso | en | en |
dc.source | Methods in Molecular Biology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85113884822&doi=10.1007%2f978-1-0716-1697-0_21&partnerID=40&md5=7c532efe1dc5a1e6a672e3eb40bd0e2d | |
dc.subject | long untranslated RNA | en |
dc.subject | messenger RNA | en |
dc.subject | microRNA | en |
dc.subject | Thy 1 membrane glycoprotein | en |
dc.subject | untranslated RNA | en |
dc.subject | long untranslated RNA | en |
dc.subject | messenger RNA | en |
dc.subject | microRNA | en |
dc.subject | assay | en |
dc.subject | computer model | en |
dc.subject | crosslinking immunoprecipitation | en |
dc.subject | embryo tissue | en |
dc.subject | gene expression profiling | en |
dc.subject | gene interaction | en |
dc.subject | half life time | en |
dc.subject | HITS-CLIP | en |
dc.subject | intracellular space | en |
dc.subject | luciferase assay | en |
dc.subject | microarray analysis | en |
dc.subject | protein degradation | en |
dc.subject | protein expression | en |
dc.subject | protein function | en |
dc.subject | protein processing | en |
dc.subject | regulatory mechanism | en |
dc.subject | RNA analysis | en |
dc.subject | RNA binding | en |
dc.subject | RNA immunoprecipitation | en |
dc.subject | gene regulatory network | en |
dc.subject | genetics | en |
dc.subject | human | en |
dc.subject | Gene Regulatory Networks | en |
dc.subject | Humans | en |
dc.subject | MicroRNAs | en |
dc.subject | RNA, Long Noncoding | en |
dc.subject | RNA, Messenger | en |
dc.subject | Humana Press Inc. | en |
dc.title | Characterizing miRNA–lncRNA Interplay | en |
dc.type | bookChapter | en |