Mostra i principali dati dell'item
Plant terpenoid metabolism co-opts a component of the cell wall biosynthesis machinery
dc.creator | Jozwiak A., Sonawane P.D., Panda S., Garagounis C., Papadopoulou K.K., Abebie B., Massalha H., Almekias-Siegl E., Scherf T., Aharoni A. | en |
dc.date.accessioned | 2023-01-31T08:29:03Z | |
dc.date.available | 2023-01-31T08:29:03Z | |
dc.date.issued | 2020 | |
dc.identifier | 10.1038/s41589-020-0541-x | |
dc.identifier.issn | 15524450 | |
dc.identifier.uri | http://hdl.handle.net/11615/74122 | |
dc.description.abstract | Glycosylation is one of the most prevalent molecular modifications in nature. Single or multiple sugars can decorate a wide range of acceptors from proteins to lipids, cell wall glycans and small molecules, dramatically affecting their activity. Here, we discovered that by ‘hijacking’ an enzyme of the cellulose synthesis machinery involved in cell wall assembly, plants evolved cellulose synthase-like enzymes (Csls) and acquired the capacity to glucuronidate specialized metabolites, that is, triterpenoid saponins. Apparently, endoplasmic reticulum-membrane localization of Csls and of other pathway proteins was part of evolving a new glycosyltransferase function, as plant metabolite glycosyltransferases typically act in the cytosol. Discovery of glucuronic acid transferases across several plant orders uncovered the long-pursued enzymatic reaction in the production of a low-calorie sweetener from licorice roots. Our work opens the way for engineering potent saponins through microbial fermentation and plant-based systems. [Figure not available: see fulltext.]. © 2020, The Author(s), under exclusive licence to Springer Nature America, Inc. | en |
dc.language.iso | en | en |
dc.source | Nature Chemical Biology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084813939&doi=10.1038%2fs41589-020-0541-x&partnerID=40&md5=438127266248d144752819b57f05036d | |
dc.subject | glucuronic acid | en |
dc.subject | glycosyltransferase | en |
dc.subject | Glycyrrhiza glabra root | en |
dc.subject | glycyrrhizic acid | en |
dc.subject | saponin derivative | en |
dc.subject | terpenoid | en |
dc.subject | cellulose | en |
dc.subject | cellulose synthase | en |
dc.subject | glucosyltransferase | en |
dc.subject | glucuronic acid | en |
dc.subject | glycosyltransferase | en |
dc.subject | plant protein | en |
dc.subject | saponin | en |
dc.subject | terpene | en |
dc.subject | amino terminal sequence | en |
dc.subject | Article | en |
dc.subject | carboxy terminal sequence | en |
dc.subject | cell wall | en |
dc.subject | controlled study | en |
dc.subject | endoplasmic reticulum | en |
dc.subject | endoplasmic reticulum membrane | en |
dc.subject | enzyme active site | en |
dc.subject | enzyme synthesis | en |
dc.subject | extracellular space | en |
dc.subject | gene cluster | en |
dc.subject | metabolic engineering | en |
dc.subject | nonhuman | en |
dc.subject | nuclear magnetic resonance | en |
dc.subject | plant metabolism | en |
dc.subject | priority journal | en |
dc.subject | protein function | en |
dc.subject | protein localization | en |
dc.subject | site directed mutagenesis | en |
dc.subject | spinach | en |
dc.subject | beet | en |
dc.subject | biosynthesis | en |
dc.subject | cell membrane | en |
dc.subject | cell wall | en |
dc.subject | gene expression regulation | en |
dc.subject | genetics | en |
dc.subject | glycosylation | en |
dc.subject | Glycyrrhiza | en |
dc.subject | mass fragmentography | en |
dc.subject | metabolism | en |
dc.subject | plant cell | en |
dc.subject | plant root | en |
dc.subject | Beta vulgaris | en |
dc.subject | Cell Membrane | en |
dc.subject | Cell Wall | en |
dc.subject | Cellulose | en |
dc.subject | Endoplasmic Reticulum | en |
dc.subject | Gas Chromatography-Mass Spectrometry | en |
dc.subject | Gene Expression Regulation, Plant | en |
dc.subject | Glucosyltransferases | en |
dc.subject | Glucuronic Acid | en |
dc.subject | Glycosylation | en |
dc.subject | Glycosyltransferases | en |
dc.subject | Glycyrrhiza | en |
dc.subject | Plant Cells | en |
dc.subject | Plant Proteins | en |
dc.subject | Plant Roots | en |
dc.subject | Saponins | en |
dc.subject | Spinacia oleracea | en |
dc.subject | Terpenes | en |
dc.subject | Nature Research | en |
dc.title | Plant terpenoid metabolism co-opts a component of the cell wall biosynthesis machinery | en |
dc.type | journalArticle | en |
Files in questo item
Files | Dimensione | Formato | Mostra |
---|---|---|---|
Nessun files in questo item. |