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Diagnostic value of immunohistochemistry for the detection of the BRAF V600E mutation in colorectal carcinoma

dc.creatorIoannou M., Papamichali R., Samara M., Paraskeva E., Papacharalambous C., Baxevanidou K., Koukoulis G.en
dc.date.accessioned2023-01-31T08:28:37Z
dc.date.available2023-01-31T08:28:37Z
dc.date.issued2016
dc.identifier.issn11070625
dc.identifier.urihttp://hdl.handle.net/11615/74054
dc.description.abstractPurpose: V600E is the most common activating BRAF mutation in colorectal carcinomas (CRCs). It is a crucial biomarker for patient selection and response to targeted therapy with BRAF V600E inhibitors. Previous studies using immunohistochemistry (IHC) have shown different results. In this study, we evaluated the IHC expression of the mutated BRAF protein in archival material from CRC specimens and correlated it with DNA sequence analysis. Methods: 51 cases of primary colon adenocarcinoma were stained with BRAF V600E-specific clone VE1 antibody against mutated BRAF protein. DNA sequence analysis was performed and the results were compared. Results: BRAF V600E protein was detected in the cytoplasm of neoplastic cells in 15 of the 51 examined cases (29.4%). The correlation between IHC staining and DNA sequence analysis showed 93.75% sensitivity and 100% specificity. Conclusions: Our data show that IHC could be used in routine clinical practice as a screening method for BRAF V600E mutant protein detection in CRC patients.en
dc.language.isoenen
dc.sourceJournal of B.U.ON.en
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84994135511&partnerID=40&md5=0dcf68c6e0f3da00c2ceaa2bccc1dc15
dc.subjectB Raf kinaseen
dc.subjectB Raf kinaseen
dc.subjectBRAF protein, humanen
dc.subjectKRAS protein, humanen
dc.subjectprotein p21en
dc.subjectageden
dc.subjectArticleen
dc.subjectcancer diagnosisen
dc.subjectcolorectal carcinomaen
dc.subjectcontrolled studyen
dc.subjectcytoplasmen
dc.subjectdiagnostic test accuracy studyen
dc.subjectdiagnostic valueen
dc.subjectDNA sequenceen
dc.subjectexonen
dc.subjectfemaleen
dc.subjectgene mutationen
dc.subjecthumanen
dc.subjectimmunohistochemistryen
dc.subjectimmunoreactivityen
dc.subjectmajor clinical studyen
dc.subjectmaleen
dc.subjectpolymerase chain reactionen
dc.subjectprotein expressionen
dc.subjectretrospective studyen
dc.subjectsensitivity and specificityen
dc.subjectsmooth muscle cellen
dc.subjectcolorectal tumoren
dc.subjectgeneticsen
dc.subjectimmunohistochemistryen
dc.subjectmutationen
dc.subjectColorectal Neoplasmsen
dc.subjectFemaleen
dc.subjectHumansen
dc.subjectImmunohistochemistryen
dc.subjectMaleen
dc.subjectMutationen
dc.subjectProto-Oncogene Proteins B-rafen
dc.subjectProto-Oncogene Proteins p21(ras)en
dc.subjectRetrospective Studiesen
dc.subjectSequence Analysis, DNAen
dc.subjectZerbinis Publicationsen
dc.titleDiagnostic value of immunohistochemistry for the detection of the BRAF V600E mutation in colorectal carcinomaen
dc.typejournalArticleen


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