Measurement and modeling of microbial growth using timelapse video

Abstract

The development of timelapse videos for the investigation of growing microbial colonies has gained increasing interest due to its low cost and complexity implementation. In the present study, a simple experimental setup is proposed for periodic snapshot acquisition of a petri dish cultivating a fungus of the genus Candida SPP, thus creating a timelapse video. A computational algorithm, based on image processing techniques is proposed for estimating the microbial population and for extracting the experimental population curves, showing the time evolution of the population of microbes at any region of the dish. Likewise, a novel mathematical population evolution modeling approach is reported, which is based on the logistic function (LF). Parameter estimation of the aforementioned model is described and visually assessed, in comparison with the conventional and widely-used LF method. The effect of the image analysis parameterization is also highlighted. Our experiments take into account different area sizes, i.e., the number of pixels in the neighborhood, to generate population curves and calculate the model parameters. Our results reveal that, as the size of the area increases, the curve becomes smoother, the signal-to-noise-ratio increases and the estimation of model parameters becomes more accurate. © 2020 by the authors.