dc.creator | Eleftheriadis T., Pissas G., Antoniadi G., Liakopoulos V., Stefanidis I. | en |
dc.date.accessioned | 2023-01-31T07:37:13Z | |
dc.date.available | 2023-01-31T07:37:13Z | |
dc.date.issued | 2016 | |
dc.identifier | 10.1007/s11255-016-1247-6 | |
dc.identifier.issn | 03011623 | |
dc.identifier.uri | http://hdl.handle.net/11615/71320 | |
dc.description.abstract | Purpose: Ischemic acute kidney injury is characterized by apoptosis of tubular epithelial cells. Proteasome plays a key role in cellular processes such as proliferation, apoptosis and inflammation. The results of animal studies about the effect of proteasome inhibitors on the course of ischemic acute kidney injury are controversial. Methods: Primary human renal tubular epithelial cells were cultured with or without the hypoxia mimetic CoCl2 and with or without the proteasome inhibitor CEP-18770 and/or the immunoproteasome inhibitor ONX-0914. The level of the proteasome subunit β5, the immunoproteasome subunits LMP7 and LMP2, the function of these proteolytic machines, HIF-1α and its transcriptional target lactate dehydrogenase-A, p53 and its transcriptional targets TP53-inducible glycolysis and apoptosis regulator and p21, and finally of activated cleaved caspase-3 were assessed by means of western blotting. Results: CoCl2 decreased the expression of β5, LMP7 and LMP2, as well as the activity of proteasome and immunoproteasome. It increased HIF-1α and its function, along with p53 and its function and induced apoptosis. CEP-18770 and ONX-0914 induced the above alterations toward the same directions as CoCl2 does. In CoCl2-treated cells, pretreatment with CEP-18770 and/or ONX-0914 potentiates the changes induced by CoCl2 alone. Conclusion: CoCl2, CEP-18770 and ONX-0914 induce apoptosis in human renal tubular epithelial cells. Importantly, proteasome or immunoproteasome inhibitors are rather toxic than beneficial in human renal tubular epithelial cells treated with the hypoxia mimetic CoCl2. © 2016, Springer Science+Business Media Dordrecht. | en |
dc.language.iso | en | en |
dc.source | International Urology and Nephrology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84975497524&doi=10.1007%2fs11255-016-1247-6&partnerID=40&md5=4ae48b69c1f8fdb767065f1d006eebb4 | |
dc.subject | caspase 3 | en |
dc.subject | cobalt chloride | en |
dc.subject | delanzomib | en |
dc.subject | hypoxia inducible factor 1alpha | en |
dc.subject | lactate dehydrogenase | en |
dc.subject | low molecular mass polypeptide 2 | en |
dc.subject | low molecular mass polypeptide 7 | en |
dc.subject | membrane protein | en |
dc.subject | onx 0914 | en |
dc.subject | proteasome inhibitor | en |
dc.subject | protein p21 | en |
dc.subject | protein p53 | en |
dc.subject | unclassified drug | en |
dc.subject | boronic acid derivative | en |
dc.subject | delanzomib | en |
dc.subject | oligopeptide | en |
dc.subject | PR-957 | en |
dc.subject | proteasome inhibitor | en |
dc.subject | threonine | en |
dc.subject | apoptosis | en |
dc.subject | Article | en |
dc.subject | cell hypoxia | en |
dc.subject | cell mediated cytotoxicity | en |
dc.subject | concentration response | en |
dc.subject | controlled study | en |
dc.subject | cytolysis | en |
dc.subject | drug potentiation | en |
dc.subject | epithelium cell | en |
dc.subject | human | en |
dc.subject | human cell | en |
dc.subject | kidney tubule epithelium | en |
dc.subject | protein analysis | en |
dc.subject | protein cleavage | en |
dc.subject | protein expression | en |
dc.subject | protein function | en |
dc.subject | Western blotting | en |
dc.subject | analogs and derivatives | en |
dc.subject | apoptosis | en |
dc.subject | cell culture technique | en |
dc.subject | cell hypoxia | en |
dc.subject | drug effects | en |
dc.subject | epithelium cell | en |
dc.subject | kidney tubule | en |
dc.subject | pathology | en |
dc.subject | Apoptosis | en |
dc.subject | Boronic Acids | en |
dc.subject | Cell Culture Techniques | en |
dc.subject | Cell Hypoxia | en |
dc.subject | Epithelial Cells | en |
dc.subject | Humans | en |
dc.subject | Kidney Tubules | en |
dc.subject | Oligopeptides | en |
dc.subject | Proteasome Inhibitors | en |
dc.subject | Threonine | en |
dc.subject | Springer Netherlands | en |
dc.title | Proteasome or immunoproteasome inhibitors cause apoptosis in human renal tubular epithelial cells under normoxic and hypoxic conditions | en |
dc.type | journalArticle | en |