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Nano-TiO2 particles impair adhesion of airway epithelial cells to fibronectin
dc.creator | Zarogiannis, S. G. | en |
dc.creator | Filippidis, A. S. | en |
dc.creator | Fernandez, S. | en |
dc.creator | Jurkuvenaite, A. | en |
dc.creator | Arnbalavanan, N. | en |
dc.creator | Stanishevsky, A. | en |
dc.creator | Vohra, Y. K. | en |
dc.creator | Matalon, S. | en |
dc.date.accessioned | 2015-11-23T10:54:50Z | |
dc.date.available | 2015-11-23T10:54:50Z | |
dc.date.issued | 2013 | |
dc.identifier | 10.1016/j.resp.2012.08.013 | |
dc.identifier.issn | 1569-9048 | |
dc.identifier.uri | http://hdl.handle.net/11615/34852 | |
dc.description.abstract | Titanium dioxide engineered nanoparticles (nano-TiO2) are widely used in the manufacturing of a number of products. Due to their size (<100 nm), when inhaled they may be deposited in the distal lung regions and damage Clara cells. We investigated the mechanisms by which short-term (1-h) incubation of human airway Clara-like (H441) cells to nano-TiO2 (6 nm in diameter) alters the ability of H441 cells to adhere to extracellular matrix. Our results show that 1 h post-incubation, there was a 3-fold increase of extracellular H2O2, increased intracellular oxidative stress as demonstrated by 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) oxidation, and a 5-fold increase of phosphor-ERK1/2 as measured by Western blotting. These changes were accompanied by a 25% decrease of H441 adherence to fibronectin (p < 0.05 compared to vehicle incubated H441 cells). Pretreatment with the ERK1/2 inhibitor U0126 for 3 h, partially prevented this effect. In conclusion, short-term exposure of H441 cells to nano-TiO2 appears to reduce adherence to fibronectin due to oxidative stress and activation of ERK1/2. (C) 2012 Elsevier B.V. All rights reserved. | en |
dc.source.uri | <Go to ISI>://WOS:000314481200034 | |
dc.subject | Airway epithelial cells | en |
dc.subject | Clara cells | en |
dc.subject | ERK1/2 | en |
dc.subject | Fibronectin | en |
dc.subject | Titanium | en |
dc.subject | nanoparticles | en |
dc.subject | Reactive oxygen species | en |
dc.subject | TITANIUM-DIOXIDE NANOPARTICLES | en |
dc.subject | ACTIVATION | en |
dc.subject | TOXICITY | en |
dc.subject | TIO2 | en |
dc.subject | NANOMATERIALS | en |
dc.subject | CYTOTOXICITY | en |
dc.subject | MICE | en |
dc.subject | SIZE | en |
dc.subject | Physiology | en |
dc.subject | Respiratory System | en |
dc.title | Nano-TiO2 particles impair adhesion of airway epithelial cells to fibronectin | en |
dc.type | journalArticle | en |
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