Development of an egg-white bioassay for monitoring biotin levels in urine and serum

dc.creator	Zarogiannis, S.	en
dc.creator	Liakopoulos, V.	en
dc.creator	Hatzoglou, C.	en
dc.creator	Vogiatzidis, K.	en
dc.creator	Salmas, M.	en
dc.creator	Stefanidis, I.	en
dc.creator	Gourgoulianis, K.	en
dc.creator	Molyvdas, P. A.	en
dc.creator	Lafis, S.	en
dc.date.accessioned	2015-11-23T10:54:49Z
dc.date.available	2015-11-23T10:54:49Z
dc.date.issued	2007
dc.identifier	10.2116/analsci.23.593
dc.identifier.issn	0910-6340
dc.identifier.uri	http://hdl.handle.net/11615/34848
dc.description.abstract	This article reports on the development of a simple and cost-effective bioassay for the detection of biotin in urine and serum, based on the very selective binding of avidin and biotin. Avidin was allowed to react without isolating it from egg white. Egg white was treated with the dye HABA, which binds to avidin. Upon subsequent treatment with biotin, HABA is released due to the high affinity of biotin to avidin. The amount of HABA released is proportional to the amount of biotin used.	en
dc.source.uri	<Go to ISI>://WOS:000246716000016
dc.subject	Chemistry, Analytical	en
dc.title	Development of an egg-white bioassay for monitoring biotin levels in urine and serum	en
dc.type	journalArticle	en

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