Development of a single-tube polymerase chain reaction assay for the simultaneous detection of Haemophilus influenzae, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus spp. directly in clinical samples
dc.creator | Xirogianni, A. | en |
dc.creator | Tzanakaki, G. | en |
dc.creator | Karagianni, E. | en |
dc.creator | Markoulatos, P. | en |
dc.creator | Kourea-Kremastinou, J. | en |
dc.date.accessioned | 2015-11-23T10:54:18Z | |
dc.date.available | 2015-11-23T10:54:18Z | |
dc.date.issued | 2009 | |
dc.identifier | 10.1016/j.diagmicrobio.2008.09.017 | |
dc.identifier.issn | 0732-8893 | |
dc.identifier.uri | http://hdl.handle.net/11615/34704 | |
dc.description.abstract | This study describes the development and evaluation of a multiplex single-tube polymerase chain reaction assay for the simultaneous detection of Haemophilus influenzae, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus spp. used as target species-specific or genus-specific genes. The assay enables the detection of 5 to 50 pg of bacterial DNA. The sensitivity of the assay was evaluated as 100% for R aeruginosa, S. aureus, and Streptococcus spp., and 94.3% for H. influenzae; the specificity was 100% for all 4 microorganisms (positive predictive value, 100%; negative predictive value, 98.2%). The assay permits rapid and accurate detection of these 4 microorganisms in a wide range of clinical samples such as whole blood, cerebrospinal, ear, pleural and ophthalmic fluids, as well as bronchoalveolar lavage and bronchial secretions. (c) 2009 Published by Elsevier Inc. | en |
dc.source | Diagnostic Microbiology and Infectious Disease | en |
dc.source.uri | <Go to ISI>://WOS:000263212100001 | |
dc.subject | Haemophilus influenzae | en |
dc.subject | Streptococcus spp. | en |
dc.subject | Pseudomonas aeruginosa | en |
dc.subject | Stalphylococcus aureus | en |
dc.subject | Multiplex PCR | en |
dc.subject | Clinical samples | en |
dc.subject | BACTERIAL-MENINGITIS | en |
dc.subject | CEREBROSPINAL-FLUID | en |
dc.subject | RAPID DETECTION | en |
dc.subject | MULTIPLEX | en |
dc.subject | PCR | en |
dc.subject | MENINGOCOCCAL DISEASE | en |
dc.subject | IDENTIFICATION | en |
dc.subject | DIAGNOSIS | en |
dc.subject | PNEUMONIAE | en |
dc.subject | GENE | en |
dc.subject | AMPLIFICATION | en |
dc.subject | Infectious Diseases | en |
dc.subject | Microbiology | en |
dc.title | Development of a single-tube polymerase chain reaction assay for the simultaneous detection of Haemophilus influenzae, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus spp. directly in clinical samples | en |
dc.type | journalArticle | en |
Fichier(s) constituant ce document
Fichiers | Taille | Format | Vue |
---|---|---|---|
Il n'y a pas de fichiers associés à ce document. |