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Th1/Th2 cytokine pattern in bronchoalveolar lavage fluid and induced sputum in pulmonary sarcoidosis
dc.creator | Tsiligianni, I. | en |
dc.creator | Antoniou, K. M. | en |
dc.creator | Kyriakou, D. | en |
dc.creator | Tzanakis, N. | en |
dc.creator | Chrysofakis, G. | en |
dc.creator | Siafakas, N. M. | en |
dc.creator | Bouros, D. | en |
dc.date.accessioned | 2015-11-23T10:51:39Z | |
dc.date.available | 2015-11-23T10:51:39Z | |
dc.date.issued | 2005 | |
dc.identifier | 10.1186/1471-2466-5-8 | |
dc.identifier.issn | 14712466 | |
dc.identifier.uri | http://hdl.handle.net/11615/33938 | |
dc.description.abstract | Background: Sarcoidosis is thought to be a T-helper type 1 cytokine (Th2 cytokine) mediated disorder. Induced sputum (IS) has been proposed as a useful non-invasive method, mainly for the assessment of the airway diseases. The aim of this study was to explore induced sputum (IS) CD4+Th1 T-lymphocyte subpopulation and to compare them with those of bronchoalveolar lavage fluid (BALF) in patients with sarcoidosis. Methods: We studied prospectively 21 patients (12 female, 9 male) of median age 46 yr (range, 25-65) with sarcoidosis and 10 normal subjects (5 female, 5 male) of median age 39 yr (range, 26-60). IS was performed with hypertonic saline solution using an ultrasonic nebulizer. BALF was performed within 10 days of IS. After stimulation of sputum lymphocytes with phorbol-myristate-acetate, we used double immunocytochemical methods to identify CD4+ IFN-γ positive and IL-4 positive cells (Th1 and Th2, respectively). Results: Sarcoidosis patients had an increased number of CD4+ -IFN-γ producing cells in IS (p = 0.003) and BALF (p = 0.01) in comparison with normal subjects. No significant differences were detected between CD4+ -IL-4 cells in BALF (p = 0.053, NS) and IS (p = 0.46, NS) between sarcoidosis patients and healthy controls. The ratio of Th1 to Th2 cells in BALF and IS was statistically different in sarcoidosis when compared with normal subjects (p = 0.007 in BALF and IS). A significant correlation was found between CD4+ IFN-γ positive cells in IS and those in BALF in sarcoidosis patients (r = 0.685, p = 0.0006). Conclusion: These data suggests that a Th1-like cytokine pattern can be observed in CD4+ T-lymphocytes in IS in patients with pulmonary sarcoidosis. Further studies are needed to explore the value of IS vs BALF in the follow-up of these patients. © 2005 Tsiligianni et al; licensee BioMed Central Ltd. | en |
dc.source.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-26844509263&partnerID=40&md5=df7d0e7aa87b91960a9b7d03dc8c1d7d | |
dc.subject | CD4 antigen | en |
dc.subject | cytokine | en |
dc.subject | gamma interferon | en |
dc.subject | interleukin 4 | en |
dc.subject | phorbol 13 acetate 12 myristate | en |
dc.subject | sodium chloride | en |
dc.subject | adult | en |
dc.subject | aged | en |
dc.subject | article | en |
dc.subject | clinical article | en |
dc.subject | controlled study | en |
dc.subject | correlation function | en |
dc.subject | cytokine production | en |
dc.subject | data analysis | en |
dc.subject | female | en |
dc.subject | follow up | en |
dc.subject | human | en |
dc.subject | human cell | en |
dc.subject | immunocytochemistry | en |
dc.subject | lung lavage | en |
dc.subject | male | en |
dc.subject | nebulizer | en |
dc.subject | prospective study | en |
dc.subject | sarcoidosis | en |
dc.subject | sputum | en |
dc.subject | statistical significance | en |
dc.subject | T lymphocyte activation | en |
dc.subject | T lymphocyte subpopulation | en |
dc.subject | Th1 cell | en |
dc.subject | Th2 cell | en |
dc.subject | CD4+ T lymphocyte | en |
dc.subject | chemistry | en |
dc.subject | comparative study | en |
dc.subject | cytology | en |
dc.subject | immunohistochemistry | en |
dc.subject | immunology | en |
dc.subject | middle aged | en |
dc.subject | Bronchoalveolar Lavage Fluid | en |
dc.subject | CD4-Positive T-Lymphocytes | en |
dc.subject | Cytokines | en |
dc.subject | Humans | en |
dc.subject | Sarcoidosis, Pulmonary | en |
dc.title | Th1/Th2 cytokine pattern in bronchoalveolar lavage fluid and induced sputum in pulmonary sarcoidosis | en |
dc.type | journalArticle | en |
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