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Chromogenic in situ Hybridization Analysis of EGFR Gene Copies in Colon Adenocarcinoma Based on Intra-Operative Imprints and Tissue Microarrays
dc.creator | Tsiambas, E. | en |
dc.creator | Rigopoulos, D. N. | en |
dc.creator | Kravvaritis, C. | en |
dc.creator | Lazaris, A. C. | en |
dc.creator | Kavantzas, N. | en |
dc.creator | Niotis, A. | en |
dc.creator | Niotis, T. H. | en |
dc.creator | Tsounis, D. | en |
dc.creator | Karameris, A. | en |
dc.creator | Patsouris, E. | en |
dc.date.accessioned | 2015-11-23T10:51:20Z | |
dc.date.available | 2015-11-23T10:51:20Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 1841-8724 | |
dc.identifier.uri | http://hdl.handle.net/11615/33880 | |
dc.description.abstract | Background: Although Epidermal Growth Factor Receptor (EGFR) over expression is a frequent event in colon adenocarcinoma (CA), identification of EGFR gene deregulation mechanisms - combined to k-ras mutations - remains the basic criterion for rational application of anti-EGFR targeted therapeutic strategies. Aim: To detect EGFR gene numerical alterations in CA based on a combination of intra-operative imprints and the corresponding tissue microarrays. Methods: 60 paraffin embedded primary CAs were cored at 1.5 mm diameter and transferred to the final microarray block. Chromogenic in situ hybridization (CISH) was performed using EGFR gene and chromosome 7 centromeric probes in the tissue microarray and also in the corresponding intra-operative imprints. Results: CISH analysis detected 4/60 (6.6%) EGFR gene amplified cases, whereas chromosome 7 aneuploidy was identified in 11/60 (18.3%) cases. Significant association was established by correlating stage to chromosome 7 (p=0.024). A high value of concordance (kappa=1) was observed comparing overall gene status based on the tissue cores and the corresponding imprints, whereas EGFR/CEN 7 copies were more numerous in imprints than in tissue microarrays (p=0.03). Conclusions: Intra-operative imprint cytology provides accurate and fast results in detecting EGFR gene/chromosome 7 centromeric signals by CISH due to the nuclear integrity and monolayer formation of the examined cells. Based on this molecular analysis, gastroenterologists and oncologists can handle those patients in a rational way regarding targeted therapies. Furthermore, chromosome 7 aneuploidy is associated with a more advanced stage in CA. | en |
dc.source.uri | <Go to ISI>://WOS:000280320500005 | |
dc.subject | Colon adenocarcinoma | en |
dc.subject | gene | en |
dc.subject | tissue microarrays | en |
dc.subject | imprints | en |
dc.subject | GROWTH-FACTOR RECEPTOR | en |
dc.subject | NEEDLE-ASPIRATION-CYTOLOGY | en |
dc.subject | CANCER CELL-LINES | en |
dc.subject | BREAST-CANCER | en |
dc.subject | COLORECTAL-CANCER | en |
dc.subject | COPY NUMBER | en |
dc.subject | EXPRESSION | en |
dc.subject | CARCINOMA | en |
dc.subject | AMPLIFICATION | en |
dc.subject | CETUXIMAB | en |
dc.subject | Gastroenterology & Hepatology | en |
dc.title | Chromogenic in situ Hybridization Analysis of EGFR Gene Copies in Colon Adenocarcinoma Based on Intra-Operative Imprints and Tissue Microarrays | en |
dc.type | journalArticle | en |
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