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Comparative Evaluation of Combined-Disk Tests Using Different Boronic Acid Compounds for Detection of Klebsiella pneumoniae Carbapenemase-Producing Enterobacteriaceae Clinical Isolates
dc.creator | Tsakris, A. | en |
dc.creator | Themeli-Digalaki, K. | en |
dc.creator | Poulou, A. | en |
dc.creator | Vrioni, G. | en |
dc.creator | Voulgari, E. | en |
dc.creator | Koumaki, V. | en |
dc.creator | Agodi, A. | en |
dc.creator | Pournaras, S. | en |
dc.creator | Sofianou, D. | en |
dc.date.accessioned | 2015-11-23T10:50:45Z | |
dc.date.available | 2015-11-23T10:50:45Z | |
dc.date.issued | 2011 | |
dc.identifier | 10.1128/jcm.00666-11 | |
dc.identifier.issn | 0095-1137 | |
dc.identifier.uri | http://hdl.handle.net/11615/33782 | |
dc.description.abstract | The accurate phenotypic detection of Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae is an increasing necessity worldwide. We evaluated the performance of boronic acid combined-disk tests using as substrate imipenem or meropenem and as inhibitor of KPC production 300 mu g aminophenylboronic acid (APBA), 600 mu g APBA, or 400 mu g phenylboronic acid (PBA). Tests were considered positive when an increase in the growth-inhibitory zone around a carbapenem disk with KPC inhibitor was 5 mm or greater of the growth-inhibitory zone diameter around the disk containing carbapenem alone. The comparison of the combined-disk tests was performed with 112 genotypically confirmed KPC-possessing Enterobacteriaceae isolates. To measure the specificity of the tests, 127 genotypically confirmed KPC-negative Enterobacteriaceae isolates that were nonsusceptible to at least one carbapenem were chosen for testing. Using disks containing imipenem without and with 300 mu g APBA, 600 mu g APBA, or 400 mu g PBA, 72, 92, and 112 of the KPC producers, respectively, gave positive results (sensitivities, 64.3%, 82.1%, and 100%, respectively). Using disks containing meropenem without and with 300 mu g APBA, 600 mu g APBA, or 400 mu g PBA, 87, 108, and 112 of the KPC producers, respectively, gave positive results (sensitivities, 77.7%, 96.4%, and 100%, respectively). Among KPC producers, the disk potentiation tests using meropenem and PBA demonstrated the largest differences in inhibition zones (P < 0.001). All combined-disk tests correctly identified 124 of the 127 non-KPC producers (specificity, 97.6%). This comparative study showed that PBA is the most effective inhibitor of KPC enzymes, and its use in combined-disk tests with meropenem may give the most easily interpreted results. | en |
dc.source | Journal of Clinical Microbiology | en |
dc.source.uri | <Go to ISI>://WOS:000293221900004 | |
dc.subject | SPECTRUM-BETA-LACTAMASE | en |
dc.subject | CLASS-A CARBAPENEMASE | en |
dc.subject | SEQUENCE TYPE 258 | en |
dc.subject | RESISTANT KLEBSIELLA | en |
dc.subject | ESCHERICHIA-COLI | en |
dc.subject | MOLECULAR EPIDEMIOLOGY | en |
dc.subject | SERRATIA-MARCESCENS | en |
dc.subject | EMERGENCE | en |
dc.subject | KPC-2 | en |
dc.subject | AMPC | en |
dc.subject | Microbiology | en |
dc.title | Comparative Evaluation of Combined-Disk Tests Using Different Boronic Acid Compounds for Detection of Klebsiella pneumoniae Carbapenemase-Producing Enterobacteriaceae Clinical Isolates | en |
dc.type | journalArticle | en |
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