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Evaluation of Boronic Acid Disk Tests for Differentiating KPC-Possessing Klebsiella pneumoniae Isolates in the Clinical Laboratory
dc.creator | Tsakris, A. | en |
dc.creator | Kristo, I. | en |
dc.creator | Poulou, A. | en |
dc.creator | Themeli-Digalaki, K. | en |
dc.creator | Ikonomidis, A. | en |
dc.creator | Petropoulou, D. | en |
dc.creator | Pournaras, S. | en |
dc.creator | Sofianou, D. | en |
dc.date.accessioned | 2015-11-23T10:50:42Z | |
dc.date.available | 2015-11-23T10:50:42Z | |
dc.date.issued | 2009 | |
dc.identifier | 10.1128/jcm.01922-08 | |
dc.identifier.issn | 0095-1137 | |
dc.identifier.uri | http://hdl.handle.net/11615/33775 | |
dc.description.abstract | The worldwide increase in the occurrence and dissemination of KPC beta-lactamases among gram-negative pathogens makes critical the early detection of these enzymes. Boronic acid disk tests using different antibiotic substrates were evaluated for detection of KPC-possessing Klebsiella pneumoniae isolates. A total of 57 genotypically confirmed KPC-possessing K. pneumoniae isolates with varying carbapenem MICs were examined. To measure the specificity of the tests, 106 non-KPC-possessing isolates (89 K. pneumoniae and 17 Escherichia coli isolates) were randomly selected among those exhibiting reduced susceptibility to cefoxitin, expanded-spectrum cephalosporins, or carbapenems. As many as 56, 53, and 40 of the non-KPC- possessing isolates harbored extended-spectrum beta-lactamases, metallo-beta-lactamases, and plasmid-mediated AmpC beta-lactamases, respectively. By use of CLSI methodology and disks containing imipenem, meropenem, or cefepime, either alone or in combination with 400 mu g of boronic acid, all 57 KPC producers gave positive results (sensitivity, 100%) whereas all 106 non-KPC producers were negative (specificity, 100%). The meropenem duplicate disk with or without boronic acid demonstrated the largest differences in inhibition zone diameters between KPC producers and non-KPC producers. By use of disks containing ertapenem, all isolates were correctly differentiated except for five AmpC producers that gave false-positive results (sensitivity, 100%; specificity, 95.3%). These practical and simple boronic acid disk tests promise to be very helpful for the accurate differentiation of KPC-possessing K. pneumoniae isolates, even in regions where different broad-spectrum beta-lactamases are widespread. | en |
dc.source | Journal of Clinical Microbiology | en |
dc.source.uri | <Go to ISI>://WOS:000263029200011 | |
dc.subject | PLASMID-MEDIATED AMPC | en |
dc.subject | C BETA-LACTAMASES | en |
dc.subject | ESCHERICHIA-COLI | en |
dc.subject | EXTENDED-SPECTRUM | en |
dc.subject | PROTEUS-MIRABILIS | en |
dc.subject | NEW-YORK | en |
dc.subject | RESISTANT | en |
dc.subject | CARBAPENEMASE | en |
dc.subject | ACINETOBACTER | en |
dc.subject | PSEUDOMONAS | en |
dc.subject | Microbiology | en |
dc.title | Evaluation of Boronic Acid Disk Tests for Differentiating KPC-Possessing Klebsiella pneumoniae Isolates in the Clinical Laboratory | en |
dc.type | journalArticle | en |
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