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Prevalence of HPV16 E1-1374 boolean AND 63nt variants in Greek women
dc.creator | Tsakogiannis, D. | en |
dc.creator | Kyriakopoulou, Z. | en |
dc.creator | Darmis, F. | en |
dc.creator | Ruether, I. G. A. | en |
dc.creator | Dimitriou, T. G. | en |
dc.creator | Orfanoudakis, G. | en |
dc.creator | Panotopoulou, E. | en |
dc.creator | Markoulatos, P. | en |
dc.date.accessioned | 2015-11-23T10:50:37Z | |
dc.date.available | 2015-11-23T10:50:37Z | |
dc.date.issued | 2014 | |
dc.identifier | 10.1002/jmv.23896 | |
dc.identifier.issn | 0146-6615 | |
dc.identifier.uri | http://hdl.handle.net/11615/33759 | |
dc.description.abstract | Recent studies have focused on sequence variation of the HPV16 E1 gene. The present study investigates the prevalence of E1-1374<^>63nt duplication in the Greek population, and the sequence variation at the 5 ' end of the E1 and E6 genes from samples that harbored this genetic alteration. Fifty HPV16 positive cervical samples, derived from Greek patients were investigated. The 5 ' end of the E1 gene was amplified through PCR and the variant amplicons were cloned, sequenced, and bioinformatically analyzed for selective pressure. The E1-1374<^>63nt duplication was identified in 24% of the examined samples, with the same prevalence in both high and low-grade cervical malignancies. The E1-1374<^>63nt duplication was linked to the European variant lineage (x(2) = 5.076, P < 0.024) and it was significantly associated with the nucleotide variation A1053C (x(2) = 23.102, P < 0.0001). Molecular evolution analyses anticipate that the E1-1374<^>63nt duplication induces functional constraints on the 5 ' end of E1 gene, and it is proposed that this duplication might not affect negatively the function or structure of the E1 protein. The E1-1374<^>63nt duplication is prevalent in the Greek population, whereas the A1053C variation might constitute a significant marker for the characterization of the E1-1374<^>63nt variant in the Greek population, thus providing significant information about viral pathogenicity. J. Med. Virol. 86:778-784, 2014. (c) 2014 Wiley Periodicals, Inc. | en |
dc.source | Journal of Medical Virology | en |
dc.source.uri | <Go to ISI>://WOS:000332675600007 | |
dc.subject | T350G variation | en |
dc.subject | E1 gene | en |
dc.subject | natural selection | en |
dc.subject | HPV16 | en |
dc.subject | E1-1374<^>63nt | en |
dc.subject | duplication | en |
dc.subject | A1053C variation | en |
dc.subject | HUMAN-PAPILLOMAVIRUS TYPE-16 | en |
dc.subject | INVASIVE CERVICAL-CANCER | en |
dc.subject | E1 PROTEIN | en |
dc.subject | DNA | en |
dc.subject | SELECTION | en |
dc.subject | GENE | en |
dc.subject | E6 | en |
dc.subject | E2 | en |
dc.subject | IDENTIFICATION | en |
dc.subject | REPLICATION | en |
dc.subject | Virology | en |
dc.title | Prevalence of HPV16 E1-1374 boolean AND 63nt variants in Greek women | en |
dc.type | journalArticle | en |
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