dc.creator | Tavoulari, S. | en |
dc.creator | Frillingos, S. | en |
dc.creator | Karatza, P. | en |
dc.creator | Messinis, I. E. | en |
dc.creator | Seferiadis, K. | en |
dc.date.accessioned | 2015-11-23T10:49:38Z | |
dc.date.available | 2015-11-23T10:49:38Z | |
dc.date.issued | 2004 | |
dc.identifier | 10.1093/humrep/deh187 | |
dc.identifier.issn | 0268-1161 | |
dc.identifier.uri | http://hdl.handle.net/11615/33590 | |
dc.description.abstract | BACKGROUND: Gonadotrophin surge-attenuating factor (GnSAF) is an as yet unidentified ovarian factor that acts on the pituitary to attenuate the pre-ovulatory LH surge. In a previous study, GnSAF bioactivity was proposed to derive, at least in part, from a C-terminal domain (95peptide) of human serum albumin (HSA). METHODS AND RESULTS: We employ here the expression-secretion system of Pichia pastoris to produce and assay selected recombinant polypeptides of HSA for GnSAF activity. We show that the C-terminal 95peptide of HSA (residues 490-585; subdomain IIIB) can be expressed from P.pastoris in secreted form and supernatants from clones expressing this polypeptide reduce the GnRH-induced LH secretion of primary rat pituitary cultures by 50-82%. When expressed in the same system, HSA domain III (residues 381-585) or full-length HSA (residues 1-585) are inactive. The bioactive subdomain IIIB is also separable from either domain III or full-length HSA on Blue Sepharose chromatography. CONCLUSIONS: Taken together, the findings highlight the putative importance of HSA subdomain IIIB as a GnSAF-bioactive entity and introduce a unique experimental tool to engineer this molecule for structure-function analysis. | en |
dc.source.uri | <Go to ISI>://WOS:000220486800014 | |
dc.subject | albumin | en |
dc.subject | GnRH | en |
dc.subject | GnSAF | en |
dc.subject | LH | en |
dc.subject | Pichia pastoris | en |
dc.subject | FOLLICLE-STIMULATING-HORMONE | en |
dc.subject | SELF-PRIMING ACTION | en |
dc.subject | INHIBITING FACTOR | en |
dc.subject | FACTOR BIOACTIVITY | en |
dc.subject | FACTOR GNSAF | en |
dc.subject | SUPEROVULATED WOMEN | en |
dc.subject | LUTEINIZING-HORMONE | en |
dc.subject | L-BETA-T2 CELLS | en |
dc.subject | FLUID | en |
dc.subject | Obstetrics & Gynecology | en |
dc.subject | Reproductive Biology | en |
dc.title | The recombinant subdomain IIIB of human serum albumin displays activity of gonadotrophin surge-attenuating factor | en |
dc.type | journalArticle | en |