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Occurrence, virulence genes and antibiotic resistance of Escherichia coli O157 isolated from raw bovine, caprine and ovine milk in Greece
dc.creator | Solomakos, N. | en |
dc.creator | Govaris, A. | en |
dc.creator | Angelidis, A. S. | en |
dc.creator | Pournaras, S. | en |
dc.creator | Burriel, A. R. | en |
dc.creator | Kritas, S. K. | en |
dc.creator | Papageorgiou, D. K. | en |
dc.date.accessioned | 2015-11-23T10:47:43Z | |
dc.date.available | 2015-11-23T10:47:43Z | |
dc.date.issued | 2009 | |
dc.identifier | 10.1016/j.fm.2009.06.002 | |
dc.identifier.issn | 0740-0020 | |
dc.identifier.uri | http://hdl.handle.net/11615/33147 | |
dc.description.abstract | The examination of 2005 raw bovine (n = 950), caprine (n = 460) and ovine (n = 595) bulk milk samples collected throughout several regions in Greece for the presence of Escherichia coli serogroup O157 resulted in the isolation of 29 strains (1.4%) of which 21 were isolated from bovine (2.2%), 3 from caprine (0.7%) and 5 from ovine (0.8%) milk. Out of the 29 E coli O157 isolates, only 12 (41.4%) could be classified as Shiga-toxigenic based on immunoassay and PCR results. All 12 Shiga-toxigenic E coli serogroup O157 isolates belonged to the E. coli O157:H7 serotype. All except one of the 12 Shiga-toxin positive isolates were stx(2)-positive, five of which were also stx(1)-positive. The remaining isolate was positive only for the stx(1) gene. All stx-positive isolates (whether positive for stx(1), stx(2) or stx(1) and stx2) were also PCR-positive for the eae and ehxA genes. The remaining 17 E. coli O157 isolates (58.6%) were negative for the presence of the H7 flagellar gene by PCR, tested negative for Shiga-toxin production both by immunoassay and PCR, and among these, only four and three strains were PCR-positive for the eae and ehxA genes, respectively. All 29 E coli O157 isolates displayed resistance to a wide range of antimicrobials, with the stx-positive isolates being, on average, resistant to a higher number of antibiotics than those which were stx-negative. (C) 2009 Elsevier Ltd. All rights reserved. | en |
dc.source.uri | <Go to ISI>://WOS:000271819300015 | |
dc.subject | Escherichia coli O157 | en |
dc.subject | STEC | en |
dc.subject | Antibiotic resistance | en |
dc.subject | Raw milk | en |
dc.subject | HEMOLYTIC-UREMIC SYNDROME | en |
dc.subject | PRODUCE SHIGA TOXIN | en |
dc.subject | BULK-TANK MILK | en |
dc.subject | DAIRY-PRODUCTS | en |
dc.subject | EWES MILK | en |
dc.subject | UNPASTEURIZED MILK | en |
dc.subject | CATTLE | en |
dc.subject | CHEESES | en |
dc.subject | STRAINS | en |
dc.subject | SPAIN | en |
dc.subject | Biotechnology & Applied Microbiology | en |
dc.subject | Food Science & Technology | en |
dc.subject | Microbiology | en |
dc.title | Occurrence, virulence genes and antibiotic resistance of Escherichia coli O157 isolated from raw bovine, caprine and ovine milk in Greece | en |
dc.type | journalArticle | en |
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