dc.creator | Siafakas, N. | en |
dc.creator | Markoulatos, P. | en |
dc.creator | Levidiotou-Stefano, S. | en |
dc.date.accessioned | 2015-11-23T10:47:14Z | |
dc.date.available | 2015-11-23T10:47:14Z | |
dc.date.issued | 2004 | |
dc.identifier | 10.1016/j.mcp.2004.06.005 | |
dc.identifier.issn | 0890-8508 | |
dc.identifier.uri | http://hdl.handle.net/11615/32982 | |
dc.description.abstract | An outbreak of aseptic meningitis was recorded in Greece during the year 2001. Detection of the clinical strains was achieved by performing reverse transcription-polymerase chain reaction (RT-PCR) on RNA isolated from cell cultures inoculated with treated faecal material from the patients. Serotypic identification of the isolates with mixed equine antisera pools followed and the RT-PCR amplicons were further studied by restriction fragment length polymorphism analysis and sequencing. Fifty-three clinical enterovirus strains were isolated from respective cases of suspected enterovirus infection, most of which showed the clinical symptoms of aseptic meningitis. Echovirus (ECV) 6 was the most frequently isolated serotype, followed by coxsackie B viruses, ECV13, poliovirus type 1 (PV1) vaccine strain and ECV30. Nucleotide sequence analysis showed the existence of different genetic groups on the basis of the 5'-untranslated region (5'-UTR) of the genome, which circulated in the population during the same time period. Different serotypes belonged to the same genetic group and vice versa. The 5'-UTR seems to be appropriate for the investigation of enterovirus evolution and epidemiology. (C) 2004 Elsevier Ltd. All rights reserved. | en |
dc.source.uri | <Go to ISI>://WOS:000224852100006 | |
dc.subject | enteroviruses | en |
dc.subject | aseptic meningitis | en |
dc.subject | 5 '-UTR | en |
dc.subject | RT-PCR | en |
dc.subject | RFLP | en |
dc.subject | seroneutralisation | en |
dc.subject | CENTRAL-NERVOUS-SYSTEM | en |
dc.subject | LENGTH-POLYMORPHISM ANALYSIS | en |
dc.subject | COXSACKIE-A | en |
dc.subject | VIRUSES | en |
dc.subject | POLIOVIRUS STRAINS | en |
dc.subject | PARALYTIC POLIOMYELITIS | en |
dc.subject | RECOMBINATION | en |
dc.subject | DIAGNOSIS | en |
dc.subject | SEQUENCE | en |
dc.subject | VACCINE | en |
dc.subject | DISEASE | en |
dc.subject | Biochemical Research Methods | en |
dc.subject | Biochemistry & Molecular Biology | en |
dc.subject | Biotechnology & Applied Microbiology | en |
dc.subject | Cell Biology | en |
dc.title | Molecular identification of enteroviruses responsible for an outbreak of aseptic meningitis; implications in clinical practice and epidemiology | en |
dc.type | journalArticle | en |