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Sequence-based genotyping HPV L1 DNA and RNA transcripts in clinical specimens
dc.creator | Satra, M. | en |
dc.creator | Vamvakopoulou, D. N. | en |
dc.creator | Sioutopoulou, D. O. | en |
dc.creator | Kollia, P. | en |
dc.creator | Kiritsaka, A. | en |
dc.creator | Sotiriou, S. | en |
dc.creator | Antonakopoulos, G. | en |
dc.creator | Alexandris, E. | en |
dc.creator | Costantoulakis, P. | en |
dc.creator | Vamvakopoulos, N. C. | en |
dc.date.accessioned | 2015-11-23T10:47:03Z | |
dc.date.available | 2015-11-23T10:47:03Z | |
dc.date.issued | 2009 | |
dc.identifier | 10.1016/j.prp.2009.07.020 | |
dc.identifier.issn | 0344-0338 | |
dc.identifier.uri | http://hdl.handle.net/11615/32909 | |
dc.description.abstract | We developed a direct sequence-based genotyping method to detect single and multiple HPV L1 DNA and RNA types in genital and dermatological specimens. Our method couples PCR amplification of a highly conserved HPV L1 segment using a broad spectrum-generic primer cocktail mix with automated sequencing of amplified PCR products, followed by GenBank sorting of sequencing data. We genotyped 5 skin and 30 cervical HPV DNA-positive specimens using this method and established its first experimentally derived working cutoff value with the aid of commercial hybridization-based techniques. We suggest that sequence-based genotyping of appropriately amplified DNA and RNA products may serve as a primary HPV detection method in dermatological specimens. It can be applied as an all-purpose genotyping method for rare HPV types not detectable by commercial hybridization-based techniques and for sorting multiple HPV infections by order of prevalence. (C) 2009 Elsevier GmbH. All rights reserved. | en |
dc.source | Pathology Research and Practice | en |
dc.source.uri | <Go to ISI>://WOS:000273493300011 | |
dc.subject | Human papilloma virus | en |
dc.subject | Sequence-based genotyping HPV L1 DNA and RNA | en |
dc.subject | transcripts | en |
dc.subject | Automated sequencing | en |
dc.subject | GenBank analysis of sequencing data | en |
dc.subject | HUMAN-PAPILLOMAVIRUS TYPES | en |
dc.subject | ADENOCARCINOMA HELA-CELLS | en |
dc.subject | INFLUENZA-B | en |
dc.subject | VIRUS | en |
dc.subject | CERVICAL-CANCER | en |
dc.subject | POTENTIAL IMPLICATIONS | en |
dc.subject | PCR | en |
dc.subject | WORLDWIDE | en |
dc.subject | Pathology | en |
dc.title | Sequence-based genotyping HPV L1 DNA and RNA transcripts in clinical specimens | en |
dc.type | journalArticle | en |
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