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Effect of porcine and ovine FSH on nuclear maturation of pig oocytes in vitro
dc.creator | Samartzi, F. | en |
dc.creator | Tsakmakidis, I. | en |
dc.creator | Theodosiado, E. | en |
dc.creator | Vainas, E. | en |
dc.date.accessioned | 2015-11-23T10:46:52Z | |
dc.date.available | 2015-11-23T10:46:52Z | |
dc.date.issued | 2008 | |
dc.identifier | 10.1111/j.1439-0531.2007.00868.x | |
dc.identifier.issn | 0936-6768 | |
dc.identifier.uri | http://hdl.handle.net/11615/32834 | |
dc.description.abstract | The effect of porcine or ovine FSH on the maturation rate of porcine oocytes and on the time course of meiotic progression was studied. Groups of 20 grade-A cumulus oocyte complexes, aspirated from slaughterhouse cycling-gilt ovaries, were cultured in vitro in 400 mu l of Modified Parker's Medium supplemented with oestrous cow serum and porcine FSH (Folltropin(R)-V, 0.50 mg/ml) or ovine FSH (Ovagen(TM), 0.44 iu/ml), in four-well dishes under mineral oil, at 38.5 degrees C, 5% CO2 in humidified air. At the end of each 3-h interval, from 3 to 42 h of culture, the nuclear status of oocytes was assessed microscopically (1000x), after fixation (methanol/acetic acid: 3/1) and orcein (2%) staining. Oocytes were classified as (i) immature (IMM), i.e. oocytes at germinal vesicle stage, germinal vesicle break down and prophase I, (ii) metaphase I (MI) and (iii) metaphase II (MII), i.e. oocytes at anaphase I, telophase I and metaphase II. Data were analysed using regression analysis, chi-square and t-test. Nuclear status was assessed in 1610 oocytes (porcine FSH: 787, ovine FSH: 823). Most of the oocytes were at MI from 24 to 33 h (porcine FSH 60.27%, ovine FSH 42.80%, p < 0.001) and at MII from 36 to 42 h (porcine FSH 80.38%, ovine FSH 67.45%, p < 0.01) of culture. Significantly higher maturation rate was observed in porcine FSH than in ovine FSH treated oocytes (86.69 +/- 12.97%, 71.34 +/- 9.86%, mean +/- SD, p < 0.05), after 42 h of culture. In conclusion, under the specific culture conditions, porcine FSH seems to support pig oocyte maturation better than ovine FSH. | en |
dc.source.uri | <Go to ISI>://WOS:000254627200005 | |
dc.subject | FOLLICLE-STIMULATING-HORMONE | en |
dc.subject | DEVELOPMENTAL COMPETENCE | en |
dc.subject | CYTOPLASMIC | en |
dc.subject | MATURATION | en |
dc.subject | MEIOTIC PROGRESSION | en |
dc.subject | CUMULUS EXPANSION | en |
dc.subject | FERTILIZATION | en |
dc.subject | INVITRO | en |
dc.subject | RESUMPTION | en |
dc.subject | COMPLEXES | en |
dc.subject | EXPOSURE | en |
dc.subject | Agriculture, Dairy & Animal Science | en |
dc.subject | Reproductive Biology | en |
dc.subject | Veterinary | en |
dc.subject | Sciences | en |
dc.title | Effect of porcine and ovine FSH on nuclear maturation of pig oocytes in vitro | en |
dc.type | journalArticle | en |
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