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Anti-mitochondrial antibody immunofluorescent titres correlate with the number and intensity of immunoblot-detected mitochondrial bands in patients with primary biliary cirrhosis
dc.creator | Rigopoulou, E. I. | en |
dc.creator | Bogdanos, D. P. | en |
dc.creator | Liaskos, C. | en |
dc.creator | Koutsoumpas, A. | en |
dc.creator | Baum, H. | en |
dc.creator | Vergani, D. | en |
dc.creator | Dalekos, G. N. | en |
dc.date.accessioned | 2015-11-23T10:46:25Z | |
dc.date.available | 2015-11-23T10:46:25Z | |
dc.date.issued | 2007 | |
dc.identifier | 10.1016/j.cca.2007.01.023 | |
dc.identifier.issn | 0009-8981 | |
dc.identifier.uri | http://hdl.handle.net/11615/32627 | |
dc.description.abstract | Background: Primary biliary cirrhosis (PBC)-specific anti-mitochondrial antibodies (AMA), routinely detected by indirect immunofluoresnce, immunofix various mitochondrial antigens by immunoblot (IB). We have investigated whether there is a relationship between IFL-determined AMA titres and the number, intensity or pattern of IB-bands. Methods: By computer-assisted imaging technology, an IB analysis using human liver mitochondrial preparation was performed in 100 AMA positive PBC samples, subdivided in 5 groups each consisting of 20 cases with very high, high, intermediate, low or borderline IFL titres and in 19 AMA-negative cases. Results: Reactivity to at least 1 mitochondrial band (range 1-12) was present in 114 (96%) cases. Reactivity to the major pyruvate dehydrogenase E2-subunit 74-kDa autoantigen positively correlated with AMA IFL titres but was absent in 25% IFL-positive cases. The intensity and number of IB-bands differed significantly among groups (p<0.001). IFL AMA titres positively correlated with the total number (r = 0.8,p<0.001) and the intensity of the IB-bands (r = 0.75, p<0.001). Conclusions: Our study indicates that IFL-determined AMA relate to the number and intensity of immunofixed bands. Reactivity to PDC-E2 does not correlate with IFL titres in a considerable proportion of patients suggesting that detection methods of AMA need to be based in the entire spectrum of the disease-specific mitochondrial antigens. (c) 2007 Elsevier B.V. All rights reserved. | en |
dc.source.uri | <Go to ISI>://WOS:000246645500016 | |
dc.subject | autoantibody | en |
dc.subject | autoinummity | en |
dc.subject | autoinumme disease | en |
dc.subject | cholestasis | en |
dc.subject | liver | en |
dc.subject | disease | en |
dc.subject | CROSS-REACTIVITY | en |
dc.subject | AUTOIMMUNITY | en |
dc.subject | PROTEINS | en |
dc.subject | ANTIGEN | en |
dc.subject | Medical Laboratory Technology | en |
dc.title | Anti-mitochondrial antibody immunofluorescent titres correlate with the number and intensity of immunoblot-detected mitochondrial bands in patients with primary biliary cirrhosis | en |
dc.type | journalArticle | en |
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