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A comparative study of immunocapture ELISA and RT-PCR for screening clinical samples from Southern Greece for human influenza virus types A and B
dc.creator | Plakokefalos, E. | en |
dc.creator | Markoulatos, P. | en |
dc.creator | Ktenas, E. | en |
dc.creator | Spyrou, N. | en |
dc.creator | Vamvakopoulos, N. C. | en |
dc.date.accessioned | 2015-11-23T10:45:44Z | |
dc.date.available | 2015-11-23T10:45:44Z | |
dc.date.issued | 2000 | |
dc.identifier.issn | 0022-2615 | |
dc.identifier.uri | http://hdl.handle.net/11615/32321 | |
dc.description.abstract | An immunocapture (IC) ELISA and reverse transcriptase (RT)-PCR assays were evaluated as screening methods for the detection of influenza virus types A and B in clinical samples collected from individuals presenting with influenza-like symptoms in Southern Greece. Standard virus isolation in embryonated hens' eggs was taken as the reference method. According to the reference method, 25 (16.7%) of the 150 clinical samples examined were infected by influenza viruses - 19 type A (H3N2) and 6 type B, The sensitivity of immunocapture ELISA was 64% and that for RT-PCR was 100%, The specificity of IC ELISA was 98% and by RT-PCR 97%, The positive diagnostic value of IC ELISA was 94% and of RT-PCR 86%, whereas the negative diagnostic values for IC ELISA and PCR were 93% and 100%, respectively. These findings confirm that RT-PCR provides significantly increased sensitivity over IC ELISA and can be of value in the management of regional influenza screening surveys conducted by the national public health services. | en |
dc.source | Journal of Medical Microbiology | en |
dc.source.uri | <Go to ISI>://WOS:000165064100011 | |
dc.subject | REVERSE TRANSCRIPTION PCR | en |
dc.subject | POLYMERASE CHAIN-REACTION | en |
dc.subject | ENZYME-IMMUNOASSAY | en |
dc.subject | RAPID DIAGNOSIS | en |
dc.subject | CELL-CULTURE | en |
dc.subject | IDENTIFICATION | en |
dc.subject | IMMUNOFLUORESCENCE | en |
dc.subject | SURVEILLANCE | en |
dc.subject | INFECTIONS | en |
dc.subject | ASSAYS | en |
dc.subject | Microbiology | en |
dc.title | A comparative study of immunocapture ELISA and RT-PCR for screening clinical samples from Southern Greece for human influenza virus types A and B | en |
dc.type | journalArticle | en |
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