Genetic polymorphism of canine cytochrome P450 2D25 gene

Abstract

Human CYP2D6 is involved in the metabolism of a number of drugs including neuroleptics, tricyclic antidepressants, β-adrenoreceptors antagonists and antiarrhythmic drugs. The corresponding canine CYP2D15 have shown similar catalytic activities. In an attempt to characterize the polymorphism of canine CYP2D15, we have cloned a segment of CYP2D15 gene from exon 5 to end of exon 7 using PCR. Sequencing and alignment of the DNA fragment produced by PCR, revealed two polymorphic sites: 1) A834T (Ile250→Phe) (CYP2D15*1) located in exon 5, and 2) A1005G (Ile307→Val) (CYP2D15*2) located in exon 6. These polymorphic sites occurred with frequency of 0.725 and 0.843, respectively. The approach described here could be used as a tool for quick assessment of canine CYP2D 15 polymorphism.