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Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Meropenem Hydrolysis Assay with NH4HCO3, a Reliable Tool for Direct Detection of Carbapenemase Activity

dc.creatorPapagiannitsis, C. C.en
dc.creatorStudentova, V.en
dc.creatorIzdebski, R.en
dc.creatorOikonomou, O.en
dc.creatorPfeifer, Y.en
dc.creatorPetinaki, E.en
dc.creatorHrabak, J.en
dc.date.accessioned2015-11-23T10:43:32Z
dc.date.available2015-11-23T10:43:32Z
dc.date.issued2015
dc.identifier10.1128/jcm.03094-14
dc.identifier.issn0095-1137
dc.identifier.urihttp://hdl.handle.net/11615/31789
dc.description.abstractA comparison of a matrix-assisted laser desorption ionization-time of flight mass spectrometric (MALDI-TOF MS) meropenem hydrolysis assay with the Carba NP test showed that both methods exhibited low sensitivity (approximately 76%), mainly due to the false-negative results obtained with OXA-48-type producers. The addition of NH4HCO3 to the reaction buffer for the MALDI-TOF MS assay dramatically improved its sensitivity (98%). Automatic interpretation of the MALDI-TOF MS assay, using the MBT STAR-BL software, generally agreed with the results obtained after manual analysis. For the Carba NP test, spectrophotometric analysis found six additional carbapenemase producers.en
dc.source.uri<Go to ISI>://WOS:000353036500041
dc.subjectCARBA NP TESTen
dc.subjectBETA-LACTAMASEen
dc.subjectRAPID DETECTIONen
dc.subjectKLEBSIELLA-PNEUMONIAEen
dc.subjectRESISTANT ENTEROBACTERIACEAEen
dc.subjectPHENOTYPIC DETECTIONen
dc.subjectPSEUDOMONASen
dc.subjectHOSPITALSen
dc.subjectEMERGENCEen
dc.subjectSPREADen
dc.subjectMicrobiologyen
dc.titleMatrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Meropenem Hydrolysis Assay with NH4HCO3, a Reliable Tool for Direct Detection of Carbapenemase Activityen
dc.typejournalArticleen


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